Molecular Testing for COVID-19: What the Clinician Should Know

Coronavirus Disease 2019 (COVID-19) is a major challenge for health systems worldwide. Molecular diagnosis is a cornerstone in the management of COVID-19. Clinicians should understand the various aspects of molecular testing that may influence their judgment and management. Cycle threshold, viral shedding, and recurrence are among the most common areas of conflict among clinicians. In this review, we discuss these areas of conflict from a clinical perspective.


INTRODUCTION
The global pandemic of Coronavirus Disease 2019 (COVID-19) is a major challenge for national and international health organizations, not only because of its rapid transmission but also because of the conflict in diagnosis and treatment.Since the onset of the outbreak in December 2019 in Wuhan, China, there has been a continuous update in diagnostic tools.Despite the continuous update and improvement of diagnostic tools, we still face many diagnostic challenges.The diagnosis of COVID-19 depends on a history of exposure to an active case, clinical presentation, chest radiography computed tomography, and laboratory diagnosis.Laboratory diagnosis is used to confirm suspected cases of COVID-19, to screen asymptomatic individuals for intimate exposure to a definite patient with COVID-19, or to aid in the diagnosis of cases with unknown respiratory illness with fever [1].Confirmation of the presence of the virus is done by detecting viral RNA in the patient's nasopharynx or other specimens using realtime Reverse Transcription Polymerase Chain Reaction (RT-PCR).Other tests such as serological antibody tests, rapid antigen detection, and other laboratory methods can support the diagnosis [2].

BASIC STRUCTURES OF SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2
Coronaviruses acquired their name because of the crown-like spikes on their surface.They are a family of positive-sense, spherical, nonsegmented, single-stranded, large (100-160 nm) RNA viruses with a genome of 26-32 kb.This group has four major subgroups (genera) of coronaviruses known as alpha, beta, gamma, and delta.The first two genera mainly infect mammals while the latter two mainly infect birds.They can cause respiratory and gastrointestinal infections [3].The recent coronavirus outbreaks were with Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus, and recently the SARS-CoV-2 pandemic; all of which belong to the beta coronaviruses [4].This group of viruses can easily adapt to any environment by mutation and recombination to survive, leading to persistent and long-term health risks.Therefore, it is necessary to recognize their virological properties to prevent their rapid spread and safety risks to humanity [5].
Severe Acute Respiratory Syndrome Coronavirus 2 is a large, 50-200 nm diameter, single-stranded RNA virus with a characteristic spherical or pleomorphic, nonsegmented envelope with characteristic crown-shape spikes or peplomers.The RNA is positive sense.It has four major structural proteins: including the spike protein (S), a glycoprotein that facilitates binding of envelope viruses to host cell angiotensin-converting enzyme 2 receptors with its S1 and S2; the small envelope glycoprotein (E), with its three variants, which is involved in viral assembly, virion release and pathogenesis; membrane glycoprotein (M), which gives shape to the viral envelope and is responsible for transmembrane nutrient transport and bud release; nucleocapsid protein (N), which is localized near the viral nucleic acid material within a capsid formed from the matrix protein and aids in packaging the viral RNA genome within the viral envelope, which is a fundamental component of viral self-assembly and replication; and several other accessory proteins (Figure 1) [6,7].The coronavirus genome encodes four or five major structural proteins: S, M, E, N, and hemagglutinin esterase.The genome of SARS-CoV-2 consists of 29,903 nucleotides, composed of four main genes encoding the major structural proteins S, E, M, and N, and another six accessory genes occupying about two-thirds of the viral genome and encoding Open Reading Frame (ORF)1a and b, ORF3a, ORF6, ORF7a, ORF7b, ORF8, and ORF10 proteins, and noncoding sequences (Figure 2) [8].
There are three well-defined variants of SARS-CoV-2 according to their genomic differences.They consist of groups with closely related lineages, referred to as A, B, and C, which differ by amino acid variations, with A being the ancestral type.The A and C types are present in appreciable percentages out of East Asia (common in Americans and Europeans) as they resist the B type which is the most common variant in Far and East Asia [9,10].

MUTATIONS IN CORONAVIRUSES
Although SARS-CoV-2 is a single-stranded RNA virus, which tends to mutate more frequently than any other virus type, unlike the other RNA viruses, it does so slowly, with an average mutation rate of one per month.Certain mutations could potentially create strains that are more deadly than others or are better at spreading the virus.For example, a gene coding a mutation of the spike  protein gives it reduced shedding ability, greater flexibility, and an advantage in transmissibility over other strains of the virus [11,12].
The virus mutates as it migrates through countries; it responds to evolutionary pressures from localized populations with different immune responses or using different antiviral drugs.This pressure leads to the emergence of different strains.Over time, this could lead to strains that are resistant to drugs, complicating the global efforts to defeat the pandemic.Due to the random nature of mutations, their effects and consequences cannot be predicted [13].Therefore, it is important to track mutation evolution even if we have an effective vaccine against COVID-19.Continuous sequencing of the virus would still be essential to understand how changes in the virus might alter its lethality, transmissibility, and the efficacy of a vaccine against all strains.Rapid sharing of data is key to understanding whether the virus is changing and how it is transmitted.

MOLECULAR TESTING FOR COVID-19
Diagnostic approaches to COVID-19 include clinical, radiological, and laboratory tools.Laboratory tests can be used for confirmation of infection, staging of disease severity, treatment monitoring, prognosis, and epidemiological surveillance (Figure 3).The two main categories of tests used include nucleic-acid-dependent and serology-dependent tests.Nucleic-acid-dependent tests immediately screen for viral RNA molecules from the patient's nasal or pharyngeal cavity, while serology-dependent tests identify the presence of the specific antibodies against SARS-CoV-2 in the patient's serum [14].Nucleic acid tests have higher detection rates in the early days of infection when the viral load is expected to be high, so that a single swab from the patient's nasopharynx may contain nearly 1 million SARS-CoV-2 particles [15].By contrast, the serological tests, which depend on the patient's response by detecting IgG and IgM antibodies, typically become positive within 5-10 days after the onset of symptoms [16].
Nucleic acid testing is the most sensitive and earliest detection method for the presence of RNA of SARS-CoV-2.Viral nucleic acid can be obtained from various types of specimens, including the upper respiratory tract (nasopharyngeal or oropharyngeal swab, aspiration of nasal secretions or nasal lavage, saliva, or midturbinate swab) or lower respiratory tract [Bronchoalveolar Lavage (BAL), tracheal aspirate, or sputum].The highest detection accuracy for SARS-CoV-2 was observed in BAL (93%), followed by sputum (72%), then nasal swabs (63%), and throat swabs (32%).
Combined throat/nasal swabs showed a similar result to nasopharyngeal swabs alone in detecting SARS-CoV-2.There is some evidence that stool, cerebrospinal fluid, plasma, urine, anal swabs, and other specimens can be used to detect SARS-CoV-2, but their diagnostic value is unclear [17][18][19][20].Samples should be collected, shipped and stored under appropriate conditions.Inadequate

LABORATORY CRITERIA FOR MOLECULAR DIAGNOSIS OF SARS-COV-2
Authorization to perform molecular tests should be limited to laboratories with molecular diagnostic capabilities that meet the criteria for performing highly complex tests under the approval of the appropriate local health authority [24].The professional staff must be well trained in the various sampling procedures from collection to real-time PCR testing techniques.The biosafety level of the laboratory should be level 2 in areas performing nonpropagative procedures (e.g., sequencing, nucleic acid amplification tests) and level 3 in areas performing propagative procedures (e.g., virus culture, isolation, or neutralization assays).The laboratory should be able to treat the patient specimens of suspected or confirmed cases during shipment as a biohazard, Category B, while being able to treat virus cultures or isolates as a biohazard, Category A, which is an infectious substance capable of causing harm to humans.The laboratory should be disinfected with appropriate disinfectants that have been shown to be effective against enveloped viruses.Phenolic compounds, quaternary ammonium compounds, hypochlorite (bleach), hydrogen peroxide, or alcohol are effective disinfectants.
All laboratory personnel should strictly adhere to infection control measures and guidelines.Laboratories should be able to report positive results promptly to the appropriate national health authorities and platforms.

POSITIVE OR NEGATIVE?
No test has 100% accuracy; therefore, each test should be evaluated for sensitivity and specificity, preferably by comparison to a gold standard.The lack of such a well-defined gold standard for COVID-19 testing makes evaluating test accuracy a major challenge.The sensitivity of the molecular test in detecting SARS-CoV-2 depends on the method and type of sample collected, as well as on the population tested.It also depends on the sensitivity of the reagent kits, equipment used, and the experience of the personnel performing the test.Many factors should be considered when selecting a particular assay, such as the cost, turnaround time, instrument throughput, availability of reagents, sensitivity, and specificity of the desired test.Consideration should also be given to the underlying population being tested (e.g., contact tracing versus diagnosis of clinical cases), the potential clinical impact, and the important public health implications if COVID-19 cases are missed [19].When a low viral load is expected, such as in healthcare professionals with no or mild symptoms, or in patients recovering from an infection, it is recommended to use diagnostic tools with high sensitivity and diagnostic yield that can best identify positive clinical specimens [25].which may indicate a high viral load.A high Ct value indicates a weak response, suggesting the presence of negligible amounts of target nucleic acid in the sample, which could represent either an infectious state or environmental contamination [30].The Ct value is the relative indicator of the amount of the target in the PCR.It gives an indirect indication of viral load (relative rather than absolute).There is a considerable debate on the acceptable Ct value as it differs according to the type of kits, manufacturer, and equipment used.Each diagnostic kit has its threshold value to define a positive or negative presence of the target molecule [22].Many factors can negatively affect the Ct value, such as the threshold of the instrument to detect fluorescence or the concentration of nucleic acids, which is affected by many conditions, including the technique of swab collection and transport, extraction procedure, pipetting technique, reagents, and kits with different primers and probes, and the presence of degraded polymerase enzyme.A qualitative positive or negative PCR is usually adequate for routine diagnosis but is not appropriate to measure the viral load.A systematic assessment of the adequacy and strength of the standard curve is essential to accurately calculate the expected viral copy number.There is still great heterogeneity and discrepancies of standard curves derived from the studies reporting Ct values from sequential dilution of samples and expected viral loads [31].
There are conflicting data on the clinical implications of the Ct value.Some studies have found that patients with severe symptoms may have a high viral load, up to 60 times that of patients with mild symptoms [32].Some researchers rely on the Ct value as a testbased decision to stop patient isolation, considering that infectivity is low in patients with Ct values >24.Moreover, they consider that positive RT-PCR results in a patient without symptoms and a Ct value of 38 on day 29 after the first positive RT-PCR are due to the presence of residual viral genomic RNA, without live virus [33].
Conversely, even if Ct value is thought to indirectly represent viral load, researchers have shown that symptomatic and asymptomatic individuals may have similar amounts of viral load [34].There are not yet enough studies correlating the Ct values with disease severity or contagiousness.High Ct values give a false sense of security, although there is not enough evidence that a higher Ct value indicates low infectivity.Ct values are typically not a quantifiable estimate of viral load and are not consistent across RT-PCR platforms; therefore, the use of Ct values to assess the status of an individual who is expected to be no longer infectious is not approved or recommended by the US Centers for Disease Control [35].Thus, we should rely on the clinical situation as an adjuvant to molecular testing.

VIRAL SHEDDING: PERSISTENT INFECTION OR REINFECTION?
Some patients may become positive after being negative by RT-PCR testing for two consecutive samples.This could be due to either persistent infection or reinfection.Persistent viral RNA material can usually be expected to have higher Ct values (i.e., lower RNA copy number) than Ct values from specimens obtained shortly before or during clinical illness.Persistent viral shedding may continue in feces after it has disappeared from respiratory secretions.Viral shedding from the respiratory tract peaks on the seventh day after infection, just before, and with the onset of symptoms [36].
Fecal viral shedding may persist for 6-10 days after the throat swabs are negative, regardless of COVID-19 severity.Fecal viral shedding may persist even after improvement of gastrointestinal symptoms including diarrhea and may persist for up to 47 days after the onset of symptoms in some patients with COVID-19 [37].Viral shedding may be irregular, erratic and not constant.A single negative smear result may be deceptive, and the test may need to be repeated.Some studies have shown that this shedding can last for several weeks.A study done by Zhou et al. [38] showed that the average duration of viral shedding was about 31 days from disease onset, and the average total duration from disease onset to discharge was about 40 days.Although rare, a recurrence of COVID-19 has been described, which could be either a reinfection or a reactivation of the disease [43][44][45].Immunity developed in response to infection with SARS-CoV-2 depends on both cell-mediated and humoral immunity.However, their precise role in clearing viral infection and protecting against reactivation or recurrence is uncertain.Induced antibodies to SARS-CoV-2 infection decrease after 2-3 months, which is a normal phenomenon that occurs after the acute infection has resolved.Recurrence may occur due to a suboptimal eradication of SARS-CoV-2 infection, which allows viral replication to flare up again.Immunodeficiency due to medication or secondary to congenital or acquired disease may play a role in impaired viral wash out and promote reactivation of SARS-CoV-2.Recurrence of COVID-19 must be distinguished from the occurrence of secondary known complications of COVID-19 such as bacterial or viral superinfection, or pulmonary embolism.It should also be distinguished from persistence of viral shedding [46].Viral culture and genomic studies capable of comparing the viral strains causing both episodes can help to distinguish between three conditions: persistent viral shedding, reinfection, or recurrence [46].
Because of the important role they play, assessment of both innate and acquired immunity and monitoring inflammatory markers would be of value in understanding the underlying pathophysiological mechanism of COVID-19 recurrence [46].Viral or bacterial coinfection should also be ruled out.Similarly, an inflammatory rebound phenomenon caused by an inappropriate immune response can potentially cause the same symptoms and signs leading to confusion with recurrence.Detection of viral RNA in all patients with a possible relapse -some of whom have a low Ctand verification of the viral strain involved in the second episode either by genomic studies or by viral cultures could help distinguish between a new episode of infection (reinfection) and either viral reactivation or an immune response-dependent inflammatory rebound phenomenon [47,48].Laboratory tests help to distinguish between viral shedding, reinfection, or inflammatory rebound.We should be careful in interpreting laboratory tests, considering the type and timing of specimen collection, and identifying intermittent and erratic viral shedding.We should also consider the clinical status of the patients and the differences in sensitivities and specificities of the various test methods.

CONCLUSION
There are many points of debate in the laboratory diagnosis of COVID-19.Since molecular diagnostic tests are the cornerstone of diagnosis, continuous updating is imperative.The clinician should not rely solely on the laboratory diagnosis, and the clinical condition of the patients should be considered.

Figure 1 |
Figure 1 | Structure of severe acute respiratory syndrome coronavirus 2. The coronaviral genome encodes four major structural proteins: S, N, M, and E; all of which are vital to create structurally complete viral particles.The E protein is the smallest of the four major structural proteins.E, envelope protein; M, matrix or membrane protein; N, nucleoprotein; S, spike protein; ssRNA, single-stranded RNA (27-31.5 kb).

Figure 2 |
Figure 2 | Schematic presentation of the genomic structure of severe acute respiratory syndrome coronavirus 2 with 5¢ untranslated region (UTR) and 3¢ UTR and coding region of coronavirus disease 2019.

Figure 3 |
Figure 3 | Schematic presentation of the diagnosis of coronavirus disease 2019.samplecollection, shipping, or storage of samples may compromise the ability of the test to identify the desired sequences[21].The RT-PCR test is considered the gold standard for laboratory diagnosis of COVID-19.However, its performance requires specific reagents, tools, and training.RT-PCR provides qualitative and quantitative detection of nucleic acid for SARS-CoV-2 from either single or pooled samples[22].

7 .Figure 4 |
Figure 4 | Cycle threshold (Ct) of reverse transcription polymerase chain reaction of severe acute respiratory syndrome coronavirus 2. Fluorescence is the intensity of fluorescence light.The threshold line is set to determine the lowest level of fluorescence light detection or the point at which the amplification reaction was able to reach a fluorescent intensity above background levels (which is set by the manufacturers).Ct value is the minimum number of cycles that can amplify the target to absorb fluorescence light.Ct value is the joint point between the amplification cycle curve and the threshold line; if the cycle number is below the threshold line, there is no Ct value.
[42]t al.[39]reported a patient in whom viral shedding continued for about 2 months from symptoms onset and for 5 weeks after she was completely asymptomatic.Male patients with COVID-19 are more prone to persistent viral shedding.Other factors that may play a role in persistent viral shedding include delayed diagnosis and hospital admission after symptom onset, use of immunosuppression, severity of illness, especially if mechanical ventilation is required, delayed antiviral therapy, and presence of other comorbidities such as coronary artery disease[40].Inflammatory markers suggestive of persistent viral shedding include low serum albumin levels, high soluble interleukin-2 receptor, and high lactate dehydrogenase levels[41].The clinical significance of persistent viral shedding after recovery and potential infectivity are not yet known as there is no obvious relationship between viral load and patient infectivity.To date, there is limited evidence that individuals with clinical recovery and persistent or recurrent shedding of viral RNA can transmit SARS-CoV-2 to others.However, it is not assured that all individuals with persistent or recurrent SARS-CoV-2 RNA shedding are no longer infectious[42].