Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Session: Physiological, Biophysical, Biochemical Bioengineering

18 articles
Proceedings Article

Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion

Yahong Huai, Shangzhong Xu
In order to study Trf 2 protein function in the sperm production, the Trf2 gene was studied and the protein function was predicted in this paper. Based on blast searches with the mRNA sequence of human Trf 2 and the bovine-EST databases, we used fragment assembly and RT-PCR technology to amplify sequence....
Proceedings Article

Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid

Shengzhao Gong, Qingsheng Chen, Shimei Li
The effect of 3-O-caffeoylquinic acid on the monophenolase and diphenolase activity was tested. The experimental results showed that the activation effected of 3-O-caffeoylquinic acid on both monophenolase and diphenolase activity .3-O-caffeoylquinic acid concentration leading 50% activity increase (AC50)...
Proceedings Article

Effects of Tonifying Kidney and Spleen, Detoxification of Throat for Th1 /Th2 of IgA Nephropathy Model Rats

Di Zou, Dongmei Zhang, Yinping Wang, Shoulin Zhang
Purposes:to investigate the effects of tonifying kidney and spleen, detoxification of throat for Th1 /Th2 of IgA nephropathy model rats.Methods : 72 rats were randomly divided into blank group, model group, Tripterygium wilfordii multi glycoside tablet group (control group), tonifying kidney and spleen,...
Proceedings Article

Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase

Ren Peng, Binwen Hong, Pan Zhou
A novel fungus which produced lipase extracellularly was isolated from soil. The strain was named after Candida tropicalis SD7 based on morphological features and ITS1-5.8S–ITS4 region sequences of ribosomal RNA. The fermentation conditions were optimized in order to improve lipase production of the...
Proceedings Article

Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells

N.N. Houreld
The extracellular matrix (ECM) provides tissue structural integrity and its synthesis plays a crucial role in wound healing. Impaired wound healing ensues following the destruction of the ECM, or the inhibition of its synthesis. An imbalance in ECM synthesis and degradation is seen in patients with diabetes....
Proceedings Article

Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to MMP-9,TIMP-1 of IgA Nephropathy Model Rat

Di Zou, Yinping Wang, Tianying Chang, Shoulin Zhang
Purpose:To study the method of tonifying kidney and spleen, detoxification of throat in treatment of IgA nephropathy. Methods:72 Wistar rats were randomly divided into blank group, model group, Lotensin group, tonifying the kidney and spleen, detoxification of method of high, medium and low dose group...
Proceedings Article

The Preparation and Research of Small Molecular Group Water

Pei Jiang, Qingshan Li, Mingyang Lian
The qualities of water are closely related with human health. Small molecular group water which contains 5-6 water molecules plays an important role to human body health. This paper reviewed the pure water was treated by the micro-nano powder of the Guhai rock to prepare a kind of small molecular group...
Proceedings Article

Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to TNF-α, IL-6 of IgA Nephropathy Model Rat

Di Zou, Yinping Wang, Dongmei Zhang, Shoulin Zhang
Purpose: To study the effect and function mechanism of Tonifying Kidney and Spleen, Detoxification of Throat method on IgA nephropathy. Methods: the 72 Wistar rats were randomly divided into blank group, model group, Lotensin group, Tonifying kidney and spleen, detoxification of throat high, medium and...
Proceedings Article

Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae

Kunli Zhao, Wei Guo, Dan Zhao, Yakun Zhang, Xiaoping Yan, Yujie Gao
A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of...
Proceedings Article

Influence of Daidzein and Daidzein Metabolites on Lifespan and Stress Resistance in Caenorhabditis Elegans

Ye Wang, Xiaolei Li, Conghui Zhang, Xiuling Wang
Caenorhabditis elegans (C. elegans) was used as an animal model to study the influence of isoflavone daidzein and daidzein metabolites, including dihydrodaidzein (DHD), O-desmethylangolensin (O-Dma) and equol, on lifespan in C. elegans at the concentration of 0.1 mmol/L. The results showed that equol...
Proceedings Article

Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens

Yi Zhang, Lixue Wen, Haiyan Bao, Yingying Nie, Yan Feng, Zhilong Xiu
To activate cryptic secondary metabolic genes is a crucial issue in the search for new natural products from microorganisms. For this purpose, in the present study, a marine fungal strain Penicillium chrysogenum WP-1 was treated with dielectric barrier discharge plasma, chemical mutagens, and their combination....
Proceedings Article

Minocycline Attenuates Kidney Injury in a Rat Model Of Streptozotocin-Induced Diabetic Nephropathy

Hongping Yuan, Dongxu Zhao, Boyin Zhang
The effects of minocycline on the development of diabetic nephropathy (DN) in streptozotocin (STZ) induced diabetic rats were evaluated in this study. The diabetes rats with diabetic nephropathy (DN) were induced by STZ (55 mg/kg) injection. The experiment included 5 groups 1) normal, 2) normal plus...
Proceedings Article

Subcostal Transversus Abdominis Plane Block for Postoperative Analgesia

Kai Li, Ruizhu Liu, Lu Chen, Guoqing Zhao, Rangjuan Cao
To evaluate the effect of subcostal transversus abdominis plane (TAP) block on postoperative analgesia and short-term prognosis. Forty patients were divided into T and N groups. Ultrasound-guided bilateral subcostal TAP block was performed by injection of ropivacaine or normal saline using patient-controlled...
Proceedings Article

Mitochondrial Toxicity of Tenofovir Dipivoxil Fumarate in HK-2 Cells

Qihui Luo, Junjun Zhao, Xinglei Feng, Zhengli Chen, Wen Zeng, Li Gong, Anchun Cheng, Yubo Shen, Chunmei Zhu
Nucleoside analogs have been shown to have mitochondrial toxicity. In vitro tests can evaluate the mechanisms and severity of the toxicity. Tenofovir dipivoxil fumarate (TDF) is a novel drug for type B hepatitis. In this study, HK-2 cells were used to evaluate the effect of TDF on mitochondrial toxicity...
Proceedings Article

The Osteoprotective Effect of Zhuanggu-Guanjie Pill in Orchidectomized Mice

Xiaomei Yuan, Yanan Bi, Zeman Yan, Hong Shi, Kun Zhou
To verify the osteoprotective effect of Zhuanggu-Guanjie (ZGGJ) in male mice. The male mice were randomly divided into 6 groups: control group (sham-operation), model group (orchidectomy group), three ZGGJ groups at doses of 0.77, 1.54 and 3.08g/kg, as well as Alendronate sodium group as positive control....
Proceedings Article

Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells

Poonam Bhadja, Kai Yu, Xiuqun Yao, Jianming Ouyang
Objective: This study aims to compare the cytotoxicity and adhesion of submicron calcium oxalate monohydrate (COM) and dehydrate (COD) crystals toward African green monkey renal epithelial cells (Vero) to reveal the mechanism of kidney stone formation at cellular level. Methods: The injury effect of...
Proceedings Article

Composition Difference of Cholesterol-, Pigment- and Mixed-type Gallstones, and Their Formation Mechanisms

Yue Zhang, Qin Kui, Cenyin Rao, Xiaojun Huang, Junfa Xue, Jianming Ouyang
Objective: The chemical compositions and formation mechanisms of different types of gallstones (cholesterol-, pigment-, and mixed-type) were studied. Methods: The components of gallstones were identified by Fourier transform infrared spectroscopy (FT-IR). The contents of cholesterol, bilirubin and bile...
Proceedings Article

Fasudil Protects Against the Myocardial Hypertrophy of Transverse Aortic Constriction Via Modulating the Expression of Caspase-3 and Bcl-2 Protein

Le Li, Hao Shang, Cailing Zhang, Houquan Tao, Jirkun Cheng
Objectives: To investigate the effects of fasudil (Fas) on myocardial hypertrophy in rats. Methods Rats were randomly divided into 5 groups: control , transverse aortic constriction (TAC) model, Fas (5mg/kg) plus TAC, TAC plus Fas(20mg/kg), and TAC plus captopril (Cap, positive drug). After administering...