Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

Session: Session 2: Genetics, Genomics and Bioinformatics

9 articles
Proceedings Article

Genetic Relationship between Rubus Parvifolius and R. Coreanus (Rubus, Rosaceae) based on Simple Sequence Repeat Markers

Yan Wang, Wen He, Jing Zhang, Tao Chen, Qing Chen, Hao-Ru Tang, Lin Liu, Xiao-Rong Wang
Rubus parvifolius L. and R. coreanus Miq. are two morphologically distinct, endemic wild brambles and sympatric in China. Genetic diversity and genetic structure of the two species and their putative hybrids were investigated by using 10 SSR markers selected from 31 markers. A total of 98 alleles were...
Proceedings Article

Transcriptome Reveals That Genic SNPs Contributes to Heterosis in Cattle

Jiang-Hong Wu, Husile Gong, Qing-Jiang Luan, Ling Xue, Hai-Lian Sun, Feng Wang
Heterosis is a powerful and valuable tool for commercial beef producers, especially cow calf operations. Despite the importance of heterosis, and its extensive genetic analysis, little understanding exists of its molecular basis. We applied RNA-Seq technology to assess transcriptome-wide allele-specific...
Proceedings Article

Genome-wide Investigation of MADS-box Members and Validation of a New Member MADS8 in Fragaria Vesca

Min Zhou, Yan Wang, Hao-Ru Tang, Qing Chen
Based on the whole genome sequence of Fragria visca, the members of the MADS gene family in strawberry genome were identified and analyzed. The MADS-box containing proteins were searched using both hidden markov model fitting with Arabidopsis thaliana proteins, and de novo prediction. 85 MADS-box protein...
Proceedings Article

Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)

Xun-Ju Liu, Yan Wang, Hao-Ru Tang, Qing Chen
To understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene CER1 was cloned from blackberry. The open reading frame of this gene was 1878 bp, encoding 625 amino acid residues with a molecular weight of 72.3 kD and a theoretical isoelectric point of 8.59....
Proceedings Article

Analysis of Codon Bias on Clostridium Perfringens Del1 Genome

Lin Chen, Ting Bai, Wei Wang, Tian-Fei Liu, Li-Li Ji, Jia-Min Zhang
Clostridium perfringens is a conditional pathogen, which widely exists in the nature water, soil, intestinal tract, and serious threat to public health and safety. In this study, the codon usage of Clostridium perfringens was examined. CDS sequences of 2744 high confidence protein coding genes were selected...
Proceedings Article

Genetic Separation of Chalkiness in the Hybrid Rice

Lu Gan, Xiao-Shu Deng, Yan Liu, Jiao Chen, Ru-Yu Tang, Li-Xia Lei, Jiang-Hong Tang, Jian-Ni Zhang, Zheng-Wu Zhao
Percentage of grains with chalkiness (PGWC) controlled by multi-genes is one of the main appearance quality of rice. The selection among the offspring using genetic separation directly influences quality improvements of hybrid rice. The present study focused on the segregation of PGWC, using Huanghuazhan...
Proceedings Article

Comparison of Protein Phosphorylation Site Prediction Tools

Yao Sun, Guo-Qing Huang, Yao Li, Jia-Ying Xue, Qiong Wu, Lei Wang
To have a better understanding of phosphorylation site prediction methods, this article made a comparison of 4 online prediction tools and submitted protein sequences to these tools for analysis. The prediction results suggestted that they play a complementary role with verification experiments in protein...
Proceedings Article

Functional Predictions of Long Noncoding RNAs by MiRNAs in Populus Euphratica Oliv.

Wei-Wei Yan, Hao Zou, Sheng-Jie Luo, Zhen-Zhong Zhou, Shao-Wei Qin, Li-Feng Zhao
Long noncoding RNAs (lncRNAs) are refer as noncoding transcript RNAs and longer than 200 nucleotides (nt) in length but shorter than 10,000 nt. LncRNAs are involved in a variety of biological processes, such as development, stress response. However, the functions of lncRNAs in plants are largely unclear....
Proceedings Article

Evidences that Intake of L-valine May Affect the Lifespan-specific Local Gene Network Pattern in Caenorhabditis Elegans

Hong-Yuan Wang, Zhi-Zhou Zhang
The purpose of this study is to preliminarily investigate whether branched-chain amino acids (BCAAs) prolong the lifespan of model organism Caenorhabditis elegans (C. elegans) through modifying the ageing-related local gene network (LGN) pattern. After synchronization adult C. elegans were fed with living...