Prevention of Toxic Coagulopathy to Ensure Life Safety in Industrialized Regions

DOI

10.2991/ichw-19.2019.25How to use a DOI?

Keywords

melatonin, lead acetate, hemostasis, rats.

Abstract

Researchers of recent years have established a direct relationship between the increase in incidence and the level of anthropogenic pollution of the environment, while emphasizing the increase in the frequency of cardiovascular pathology and thrombohemorrhagic complications among the population living in areas ecologically unfavorable for lead. In this regard, it should be considered that the prevention of toxic coagulopathy is an important mechanism for ensuring life safety in industrialized regions. The purpose of this work was to study the preventive effect of melatonin on the hemostasis system indicators, the state of lipid peroxidation and antioxidant protection during chronic lead intoxication. Lead acetate solution was injected to Wistar rats at a dose of 40 mg/kg through a tube into the stomach, along with intragastric administration of a solution of melatonin at a dose of 5 mg/kg for two weeks, one and two months. Determined ADP-platelet aggregation, fibrinogen content, activated partial thromboplastin time, prothrombin time, the relative time of the polymerization of fibrin monomers, the activity of antithrombin III, while spontaneous euglobulin lysis, the concentration of soluble fibrin monomer complexes using standard techniques "technology standard", Russia. The level of D-dimers was determined by the reagents kit "INNOVANCE D-dimer", Siemens. To assess the intensity of lipid peroxidation processes, we determined the content of malondialdehyde in erythrocytes, hydroperoxides (diene conjugates and diene ketones) in blood plasma, superoxide dismutase activity, and catalase activity in erythrocytes (Solar-300, Belarus). Studies have shown that prophylactic administration of melatonin helps to reduce the severity of pathological changes in the parameters of vascular-platelet and coagulation hemostasis, anticoagulant and fibrinolytic systems, helps to reduce the level of thrombinemia. The revealed changes correlated with the restoration of the level of lipid peroxidation products and the activity of antioxidant blood enzymes. The results of the study allow us to recommend the use of melatonin for the optimization of therapeutic and preventive measures and the development of methods for the correction of hemostatic disorders when exposed to lead compounds on the body.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - E.M. Gagloeva
AU  - T.V. Moldovan
AU  - V.B. Brin
AU  - N.V. Botsieva
PY  - 2019/10
DA  - 2019/10
TI  - Prevention of Toxic Coagulopathy to Ensure Life Safety in Industrialized Regions
BT  - Proceedings of the International Conference on Health and Well-Being in Modern Society (ICHW 2019)
PB  - Atlantis Press
SP  - 101
EP  - 105
SN  - 2468-5739
UR  - https://doi.org/10.2991/ichw-19.2019.25
DO  - 10.2991/ichw-19.2019.25
ID  - Gagloeva2019/10
ER  -