Efficient Protocol for Introducing Regeneration of Adventitious Buds from Hypocotyl Explants in Jatropha curcas L.
- DOI
- 10.2991/icmse-16.2016.14How to use a DOI?
- Keywords
- Jatropha curcas; Hypocotyl explant; Regeneration; Rooting; Thidiazuron,.
- Abstract
A new strategy of high concentration short duration treatment of Thidiazuron (TDZ) for enhancing adventitious buds formation from hypocotyl explants were attempted in Jatropha curcas. The best shoot buds induction (81.91%) and number of buds per explant (10.16) were obtained from hypocotyl explants treated with 20 mg/L TDZ solution for 20 min, followed by 30 days of culture on hormone-free MS medium. The elongated shoots initiated roots to become intact plantlets in rooting medium containing indole-3-butyric acid (IBA) and L-glutamine (Gln), and the results showed that the initiation and growth of roots were effectively stimulated with supplementing 16 mg/L Gln into the rooting medium, and having the best rooting rate (51.39%). After acclimatization, an intact plantlet could usually be gained at 60 days of culture by using the culture protocol described in this study.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Ying Liu AU - Su-Fang Niu AU - Jian-Nong Lu AU - Sen Chen AU - Ya-Fei Wang AU - Hong-Bo Zhu AU - Lin-Feng Li AU - Yu-Zhen Shi AU - Xue-Gui Yin PY - 2016/12 DA - 2016/12 TI - Efficient Protocol for Introducing Regeneration of Adventitious Buds from Hypocotyl Explants in Jatropha curcas L. BT - Proceedings of the 4th 2016 International Conference on Material Science and Engineering (ICMSE 2016) PB - Atlantis Press SP - 76 EP - 84 SN - 2352-5401 UR - https://doi.org/10.2991/icmse-16.2016.14 DO - 10.2991/icmse-16.2016.14 ID - Liu2016/12 ER -