Artery Research

Volume 20, Issue C, December 2017, Pages 95 - 96

P141 COMPARISON BETWEEN TECHNIQUES OF EVALUATION MICROVASCULAR MORPHOLOGY: THE GOLD-STANDARD LOCALLY INVASIVE MICROMYOGRAPHY VS. THREE NON-INVASIVE TECHNIQUES. PRELIMINARY DATA

Authors
C. De Ciuceis1, S. Caletti1, M.A. Coschignano1, C. Rossini1, S. Duse2, F. Docchio3, S. Pasinetti3, F. Zambonardi3, F. Semeraro2, G. Sansoni4, C. Agabiti Rosei1, P. Pileri1, E. Agabiti Rosei1, D. Rizzoni1, 5
1Clinica Medica, Department of Clinical and Experimental Sciences, University of Brescia, Italy
2Chair of Ophtalmology, University of Brescia, Italy
3Department of Mechanical and Industrial Engineering, University of Brescia, Brescia, Italy
4Department of Information Engineering, University of Brescia, Brescia, Italy
5Istituto Clinico Città di Brescia, Division of Medicine, Brescia, Italy
Available Online 6 December 2017.
DOI
10.1016/j.artres.2017.10.153How to use a DOI?
Abstract

Objective: The gold standard technique of evaluation microvascular morphology in human is generally considered the measure of media to lumen ratio (M/L) of subcutaneous small vessels obtained by local biopsies and evaluated by wire or pressure micromyography. However, non-invasive techniques for the evaluation of retinal arterioles were recently proposed, in particular two approaches seem to provide interesting information: Scanning Laser Doppler Flowmetry (SLDF) and adaptive optics (AO); both of them provide an estimation of the wall to lumen ratio (WLR) of retinal arterioles. A non-invasive measurement of basal and total capillary density may be obtained by videomicroscopy/capillaroscopy. No direct comparison of the non-invasive techniques in the same population was previously performed, in particular AO was never validated against micromyography.

Methods: In the present study, we enrolled 12 normotensive subjects and 8 hypertensive patients undergoing an election surgical intervention; (11/20 were severely obese). All patients underwent a biopsy of subcutaneous fat during surgery. Subcutaneous small resistance artery structure was assessed by wire myography and the M/L was calculated. WLR of retinal arterioles was obtained by SLDF and AO (SLDF, Heidelberg Engineering, Heidelberg, Germany and RTX-1, Imagine Eyes, Orsay, France). Functional (basal) and structural (total) microvascular density were evaluated by capillaroscopy before and after venous congestion.

Results: The results are summarized in the Table (slope of the relation: p < 0.01 RTX-1 vs. SLDF).

Basal capillary density in the nailfold/M/L Total capillary density in the forearm / ML Basal capillary density in the dorsum of the finger / M/L Total capillary density in the dorsum of the finger /ML
Correlation coefficients (n = 20) 0.53, r2 = 0.28, p < 0.05 0.50, r2 = 0.25, p < 0.05 0.17, r2 = 0.29, p = NS 0.34, r2 = 0.12, p = NS





W/L retinal arterioles (SLDF)/ M/L W/L retinal arterioles (RTX- 1)/M/L W/L retinal arterioles (SLDF)/ W/L retinal arterioles (RTX-1)





Correlation coefficients (n = 20) 0.54, r2 = 0.29, p < 0.01 0.90, r2 = 0.81, p < 0.001 0.71, r2 = 0.50, p < 0.001

Conclusions: Our data suggest that AO has a substantial advantage over SLDF in terms of evaluation of microvascular morphology, since it is more closely correlated with the M/L of subcutaneous small arteries, considered a gold-standard approach.

Open Access
This is an open access article distributed under the CC BY-NC license.

Journal
Artery Research
Volume-Issue
20 - C
Pages
95 - 96
Publication Date
2017/12/06
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2017.10.153How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - C. De Ciuceis
AU  - S. Caletti
AU  - M.A. Coschignano
AU  - C. Rossini
AU  - S. Duse
AU  - F. Docchio
AU  - S. Pasinetti
AU  - F. Zambonardi
AU  - F. Semeraro
AU  - G. Sansoni
AU  - C. Agabiti Rosei
AU  - P. Pileri
AU  - E. Agabiti Rosei
AU  - D. Rizzoni
PY  - 2017
DA  - 2017/12/06
TI  - P141 COMPARISON BETWEEN TECHNIQUES OF EVALUATION MICROVASCULAR MORPHOLOGY: THE GOLD-STANDARD LOCALLY INVASIVE MICROMYOGRAPHY VS. THREE NON-INVASIVE TECHNIQUES. PRELIMINARY DATA
JO  - Artery Research
SP  - 95
EP  - 96
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.153
DO  - 10.1016/j.artres.2017.10.153
ID  - DeCiuceis2017
ER  -