Comparison of the Influence of Trypsin on the Number of Colonies Lactobacillus spp. on the Soy Milk and Cow’s Milk Medium

DOI

10.2991/ahsr.k.210723.018How to use a DOI?

Keywords

lactobacillus spp, soy milk, cow milk, trypsin

Abstract

Soy milk has high carbohydrate levels, so soy milk can be used as an alternative medium for the growth of Lactobacillus spp. In addition, in vivo, to be able to play an optimal role, Lactobacillus spp. must be able to survive the influence of enzymes in the digestive tract, including the trypsin enzyme. This research is an experimental laboratory that aims to compare the effect of trypsin addition on the number of Lactobacillus spp colonies using the TPC (Total Plate Count) method on soy milk and cow milk media. There are four treatment groups namely soy milk and cow milk with and without the addition of trypsin enzymes, each with 3 repetitions. Calculation of the number of colonies was carried out at the 36th hour. Data were analysed statistically using the t-independent test. The results showed that the average number of colonies of Lactobacillus spp. in soy milk is higher than cow milk, with or without the addition of trypsin. Amount of bacterial colony in the medium of soy milk is 2.5 x1018 CFU/ml, while in cow milk 4.5 x 1012CFU/ml. The percentage average of Lactobacillus spp mortality in soy milk is lower (54.64%) than cow milk (73.04%). There was a significant difference between the percentage of Lactobacillus spp deaths. in the media of soy milk and cow milk with p-value = 0.00 (p <0.05). The conclusion of this study shows that soymilk media is better at growing Lactobacillus spp. and more resistant to trypsin enzymes when compared to cow milk.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Eka Noneng Nawangsih
AU  - Ana Mariana
AU  - Teja Koswara
PY  - 2021
DA  - 2021/07/24
TI  - Comparison of the Influence of Trypsin on the Number of Colonies Lactobacillus spp. on the Soy Milk and Cow’s Milk Medium
BT  - Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
PB  - Atlantis Press
SP  - 72
EP  - 75
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210723.018
DO  - 10.2991/ahsr.k.210723.018
ID  - Nawangsih2021
ER  -