Isolation of Good-Quality RNA from Rosa chinensis, Rich in Secondary Metabolites

DOI

10.2991/bbbs-18.2018.43How to use a DOI?

Keywords

Rosa chinensis, RNA Isolation, CHS gene, CTAB.

Abstract

Rosa chinensis is being widely investigated because of its extremely high ornamental value and commercial value. Scientists have entered the molecules for its researching level. Extraction of high quality RNA is a primary and key step towards undertaking molecular biology experiments, which is often affected by contaminants, such as pigments, polyphenols, proteins, plant secondary metabolites, and genomic DNA from plant tissues. Here the authors build a new protocol suitable for isolating high-quality RNA from the petals of Rosa chinensis. Based on a CTAB method, the quality of RNA is very pure with little contaminants and makes a figure in gel electrophoresis. After RT-PCR, the 860 bp sequence of chalcone synthase (CHS) gene was obtained to identify the quality of total RNA from Rosa chinensis. In these experiments, The RNA isolated by the improved CTAB method is quite excellent.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yong Zhang
AU  - Yunting Zhang
AU  - Haoru Tang
PY  - 2018/04
DA  - 2018/04
TI  - Isolation of Good-Quality RNA from Rosa chinensis, Rich in Secondary Metabolites
BT  - Proceedings of the 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018)
PB  - Atlantis Press
SP  - 202
EP  - 204
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbbs-18.2018.43
DO  - 10.2991/bbbs-18.2018.43
ID  - Zhang2018/04
ER  -