Preparation of Soybean Oil Corn Starch Ester by Using Immobilized Lipase
- DOI
- 10.2991/emcm-15.2016.25How to use a DOI?
- Keywords
- Soybean oil corn starch ester Novozym 435 lipase Solvent-free system; Substituting degree
- Abstract
The soybean oil starch ester was synthesized by using Novozym435 lipase as biocatalyst, corn starch and soybean oil as substrate in solvent-free system. In order to improve the reaction activity of starch, the corn starch was pretreated using NaOH/Urea/H2O solution. The optimum pretreatment reaction conditions of enzymatic esterification of soybean oil with pretreatment starch were water activity<0.01, reaction temperature was 60 , reaction time was 30h, the ratio of soybean oil to pretreatment starch was 6 (w/w) and the amount of lipase was 7%. Under this condition the degree of substitute of soybean oil with pretreatment starch was 0.0302. The solubility, emulsifying and freeze-thawing stability of soybean oil starch ester increased with the increase of its degree of substitute, but the transparency of soybean oil starch ester decreased with its degree of substitute. The transparency of soybean oil starch ester was improved in the presence of sucrose, but decreased in the presence of sodium chloride and citric acid.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Zhengyang Wang AU - Jiaying Xin AU - Luoyun Zheng AU - Quanhui Li AU - Lirui Sun PY - 2016/02 DA - 2016/02 TI - Preparation of Soybean Oil Corn Starch Ester by Using Immobilized Lipase BT - Proceedings of the International Conference on Electronics, Mechanics, Culture and Medicine PB - Atlantis Press SP - 130 EP - 135 SN - 2352-538X UR - https://doi.org/10.2991/emcm-15.2016.25 DO - 10.2991/emcm-15.2016.25 ID - Wang2016/02 ER -