Micropropagation of Ten Genotypes of Sugarcane Mutants on Media Containing BA and Thidiazuron

Sukendah Sukendah; Ragapadmi Purnamaningsih; Rossyda Priyadarshini

doi:10.2991/978-94-6463-168-5_4

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Micropropagation of Ten Genotypes of Sugarcane Mutants on Media Containing BA and Thidiazuron

Authors

Sukendah Sukendah¹^{, 3}^{, *}, Ragapadmi Purnamaningsih², Rossyda Priyadarshini¹^{, 3}

¹University of Pembangunan Nasional Veteran Jawa Timur, Jl Raya Rungkut Madya, Surabaya, East Java, Indonesia

²National Research and Innovation Agency, Research Center for Hortikultural and Estate Crops, Bogor, Indonesia

³Center Innovation of Appropriate Technology for Lowland and Coastal Food, Surabaya, East Java, Indonesia

^*Corresponding author. Email: sukendah@upnjatim.ac.id

Corresponding Author

Sukendah Sukendah

Available Online 23 May 2023.

DOI: 10.2991/978-94-6463-168-5_4 How to use a DOI?
Keywords: clonal propagation; Saccharum officinarum; multiplication; growth regulator
Abstract: Mutation induction in sugarcane by gamma irradiation has produced 10 mutant clones. Each mutant clone needs to be reproduced before further release. Multiplication of mutant clones is done in-vitro to produce true-to-type mutant sugar cane seeds. Four in-vitro media containing BA (Benzylaminopurine) and Thidiazuron were designed to obtain the right media for each sugarcane mutant. The medium used was basic Murashige Skoog (MS) medium containing 100 mg/l PVP and 3% sucrose, with the addition of BA (1 and 3 mg/l) and thidiazuron (0 and 0.5 mg/l). The explants used were shoots from the ten genotypes of the sugarcane mutants, measuring ±0.5 cm. The study used a completely randomized design with observational variables: shoot height, number of shoots, number of roots, visual shoots and roots. The results showed that growth media containing 3 mg/l BA and 0.5 mg/l thidiazuron was the best medium for multiplication of mutant sugarcane. Growth regulator auxin NAA was more effective in inducing the formation of sugarcane roots. Clones K10 and K8 had the best shoot multiplication ability compared to other mutant clones.
Copyright: © 2023 The Author(s)
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 3rd International Conference on Agriculture (ICA 2022)
Series: Advances in Biological Sciences Research
Publication Date: 23 May 2023
ISBN: 10.2991/978-94-6463-168-5_4
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-168-5_4 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Sukendah Sukendah
AU  - Ragapadmi Purnamaningsih
AU  - Rossyda Priyadarshini
PY  - 2023
DA  - 2023/05/23
TI  - Micropropagation of Ten Genotypes of Sugarcane Mutants on Media Containing BA and Thidiazuron
BT  - Proceedings of the 3rd International Conference on Agriculture (ICA 2022)
PB  - Atlantis Press
SP  - 20
EP  - 28
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-168-5_4
DO  - 10.2991/978-94-6463-168-5_4
ID  - Sukendah2023
ER  -

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