Proceedings of the 3rd International Conference on Agriculture (ICA 2022)

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Antioxidant and Anticholesterol Activity of Chitooligosaccarides Hydrolyzed of Golden Apple Snail (Pomacea canaliculata) Shell Using Various Types of Enzyme

Authors
Dedin Finatsiyatull Rosida1, 2, *, Jariyah1, 2, Sekar Ayu Larasati1, 2, Teeradate Kongpichitchoke3, Anugerah Dany Priyanto1, 2, Andre Yusuf Trisna Putra1, 2
1Department of Food Technology, Faculty of Engineering, University of Pembangunan Nasional Veteran Jawa Timur, Jl Raya Rungkut Madya, Surabaya, 60294, East Java, Indonesia
2Center Innovation of Appropriate Technology for Lowland and Coastal Food, Surabaya, East Java, Indonesia
3Faculty of Biotechnology, Assumption University, Bangkok, Thailand
*Corresponding author. Email: dedin.tp@upnjatim.ac.id
Corresponding Author
Dedin Finatsiyatull Rosida
Available Online 23 May 2023.
DOI
10.2991/978-94-6463-168-5_8How to use a DOI?
Keywords
Chittin; Chitosan; Deacetylation; Golden Snail
Abstract

Chitosan (β1-4 N-acetyl D-glucosamine) is a chitin derivative compound which is obtained through the deacetylation stage, namely the process of releasing the acetamide group in chitin (CH3CONH) so that it becomes an amine group (NH2) in chitosan. The manufacture of chitosan is carried out through 3 stages, namely the process of deproteination, demineralization, and deacetylation. Chitosan has many functional properties because it has a reactive amine group (-NH2), some of its functional properties are antioxidant and anti-cholesterol. However, chitosan is a water insoluble compound, so its use is limited, especially in the food and medical fields. Therefore, chitosan needs to reduce its molecular weight by hydrolysis. Enzymatic hydrolysis is an alternative way that is safe and can be easily controlled. Completely Randomized Design (CRD) was used as the study design with two factors, namely the type of enzyme (cellulase, α-Amylase, and lipase) and concentration (0.1%; 0.5%; and 0.9%). The research showed that there were significant differences in the factors used on the molecular weight, enzymatic activity, antioxidant activity, and anticholesterolemic activity. The best treatment based on the results of this study was sample A1B3, namely chitooligosaccharide samples hydrolyzed using cellulase enzymes, with a concentration of 0.9%, a yield of 85.33% was obtained, with a molecular weight of 13.58 kDa, enzyme activity of 0.95 U/ml, antioxidant activity of 15.97%, and total cholesterol of 44.55 mg/dl (percentage of cholesterol reduction of 55.45%).

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Agriculture (ICA 2022)
Series
Advances in Biological Sciences Research
Publication Date
23 May 2023
ISBN
10.2991/978-94-6463-168-5_8
ISSN
2468-5747
DOI
10.2991/978-94-6463-168-5_8How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Dedin Finatsiyatull Rosida
AU  - Jariyah
AU  - Sekar Ayu Larasati
AU  - Teeradate Kongpichitchoke
AU  - Anugerah Dany Priyanto
AU  - Andre Yusuf Trisna Putra
PY  - 2023
DA  - 2023/05/23
TI  - Antioxidant and Anticholesterol Activity of Chitooligosaccarides Hydrolyzed of Golden Apple Snail (Pomacea canaliculata) Shell Using Various Types of Enzyme
BT  - Proceedings of the 3rd International Conference on Agriculture (ICA 2022)
PB  - Atlantis Press
SP  - 60
EP  - 73
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-168-5_8
DO  - 10.2991/978-94-6463-168-5_8
ID  - Rosida2023
ER  -