Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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In Vitro Selection and Total Phenol Analysis of Moon Orchid [Phalaenopsis amabilis (L.) Bl.] Results of Induced With Fusaric Acid

Authors
Endang Nurcahyani1, *, Risma Rasmani2, Hardoko Insan Qudus3
1Applied Biology Study Program, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
2Department of Agrotechnology, Faculty of Agriculture, Megou Pak Tulang Bawang University, Tulang Bawang, Lampung, Indonesia.
3Chemistry Study Program, Faculty of Mathematics and Natural Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia
*Corresponding author email: endang.nurcahyani@fmipa.unila.ac.id
Corresponding Author
Endang Nurcahyani
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.079How to use a DOI?
Keywords
Fusaric acid; In vitro; Phalaenopsis amabilis; Total phenol
Abstract

Phalaenopsis amabilis (L.) Bl. is an orchid that is included in the list of endangered plant species. The Fusarium wilt disease on P. amabilivirala vital disease caused by Fusarium oxysporum, which of one constraint and up to now days is not well yet managed. Disease control that does not cause negative impacts can be done by using superior cultivars that are resistant to Fusarium oxysporum infection, through in vitro selection on medium with the addition of selective concentration of fusaric acid. The purpose of this study was to determine the concentration of fusaric acid that was tolerant to P. amabilis plantlets and to analyze the total phenol content of plantlets after fusaric acid was induced with various concentrations. This study used a completely randomized design with one factor, with 5 levels of fusaric acid concentration, namely 0 ppm, 30 ppm, 60 ppm, 90 ppm, and 120 ppm. Data analysis used Analysis of Variance with a significance level of 5% and further test with Tukey’s test at a significance level of 5%. The results showed that: the concentration of fusaric acid that was tolerant to P. amabilis plantlets was 120 ppm; there was an increase in total phenol content along with an increase in fusaric acid concentration, an increase in total phenol content from 1.54 mg/g at a concentration of 0 ppm (control) increased to 2.0 mg/g at a concentration of 30 ppm, followed by 2.64 mg /g at a concentration of 60 ppm, 3.48 mg/g at 90 ppm and 4.27 mg/g at a concentration of 120 ppm. This proves that the higher the concentration of fusaric acid, the higher the total phenol content produced.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.079
ISSN
2468-5747
DOI
10.2991/absr.k.220406.079How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Endang Nurcahyani
AU  - Risma Rasmani
AU  - Hardoko Insan Qudus
PY  - 2022
DA  - 2022/05/02
TI  - In Vitro Selection and Total Phenol Analysis of Moon Orchid [Phalaenopsis amabilis (L.) Bl.] Results of Induced With Fusaric Acid
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 558
EP  - 564
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.079
DO  - 10.2991/absr.k.220406.079
ID  - Nurcahyani2022
ER  -