Effect of UV-B Radiation Intensity on Hsp70, mtHsc70-1, and cpHsc70-2 Gene Expression in Synedrella nodiflora (L.) Gaertn. Leaf Under Field Conditions
- 10.2991/absr.k.220406.035How to use a DOI?
- cpHsc70-2; gene expression; Hsp70; mtHsc70-1; Synedrella nodiflora; UV-B radiation
Increased expression of genes encoding Heat Shock Protein 70 (Hsp70) is one of the plant defense responses against UV-B stress. Synedrella nodiflora may be tolerant to relatively high UV-B intensity. The research has been conducted to compare expression levels of genes encoding cytosolic, mitochondrial, and plastid Hsp70 (Hsp70, mtHsc70-1, and cpHsc70-2) in S. nodiflora leaves against environmental conditions with different UV-B radiation intensities in Faculty of Mathematics and Natural Sciences campus area, Universitas Indonesia, Depok. Alpha Tubulin (TUB) was used as a reference gene to normalize the cDNA quantity of each Hsp70 gene. The levels of relative gene expression were analyzed using the Pfaffl method. Average UV-B intensity in control, shaded, and open locations were 18.4 ± 0.1, 44.1 ± 0.6, and 260.1 ± 78.3 mW/m2, respectively. Expression levels of Hsp70 and cpHsc70-2 were relatively higher in leaves collected from shaded locations, while mtHsc70-1 was relatively lower. Inversely, expression levels of Hsp70 and cpHsc70-2 were relatively lower in leaves collected from open locations, while mtHsc70-1 was relatively higher. S. nodiflora in an open location may have acclimatized to environmental conditions with relatively higher temperature, light intensity, and UV-B intensity. This acclimatization caused S. nodiflora in an open location to experience an increase in minimum temperature, light intensity, and UV-B intensity required to induce an increase in Hsp70 and cpHsc70-2 gene expression. The Hsp70, mtHsc70-1, and cpHsc70-2 genes have different expression patterns in different environmental conditions, such as different UV-B radiation intensities.
- © 2022 The Authors. Published by Atlantis Press International B.V.
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Cite this article
TY - CONF AU - Maria Celinna AU - Andi Eko Maryanto AU - Andi Salamah PY - 2022 DA - 2022/05/02 TI - Effect of UV-B Radiation Intensity on Hsp70, mtHsc70-1, and cpHsc70-2 Gene Expression in Synedrella nodiflora (L.) Gaertn. Leaf Under Field Conditions BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 246 EP - 254 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.035 DO - 10.2991/absr.k.220406.035 ID - Celinna2022 ER -