Determination of Lectin Genes in Superior Mutant of Rodent Tuber Bogor Accession (Typhonium flagelliforme) Based on PCR Amplification

DOI

10.2991/absr.k.220406.039How to use a DOI?

Keywords

Anticancer; Lectin gene; Specific primer; Superior mutant rodent tuber

Abstract

Rodent tuber (Typhonium flagelliforme) is one of the Indonesian herbs that has not yet been developed as an anticancer drug. The local name rodent tuber plant has produced superior mutant strains with higher levels and bioactive compounds. Compared to the mother plant, GCMS analysis detected four new bioactive compounds. A superior mutant rodent tuber ethanol extract was tested in vitro against MCF7 (breast) cancer cells. In previous studies, extracts from different rodent tuber varieties showed cytotoxic effects on MCF7 cancer cells at much lower IC₅₀ doses and up to 10-fold potency than mother plants. The development of the lineage of the rodent tuber plant Bogor has morphological and molecular marker characteristics. However, it is unclear whether the activity of the lectin gene is associated with Bogor’s accession. The purpose of this study was to validate specific primer amplifications associated with lectin gene characteristics in the Bogor lineage of rodent tubers. This technique uses PCR amplification with specific primers that encode the lectin gene. All mutants and control plants were successfully amplified in sizes in the 500 bp range. The PCR products continued to be sequenced and showed significant results with differences in DNA sequences (from the 5’end reading) in the three regions 59 bp, 183 bp, and 211 bp. It was confirmed that the superior mutant rodent tuber plant with high anti-cancer compound had different sequences in the control and the mutant plant with low anti-cancer compound. Based on the results, further sequence analysis studies should be conducted on the sequence differences between the rodent tuber control plant and the superior mutant plant. Information about lectin compounds in the Bogor lineage of rodent tubers can be used as the basis for developing healthcare products, especially cancer treatments.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Nesti Fronika Sianipar
AU  - Reflinur Reflinur
AU  - Muhammad Dzulkifly Ashan
AU  - Khoirunnisa Assidqi
AU  - Dwityantari Widyaningrum
AU  - Ragapadmi Purnamaningsih
PY  - 2022
DA  - 2022/05/02
TI  - Determination of Lectin Genes in Superior Mutant of Rodent Tuber Bogor Accession (Typhonium flagelliforme) Based on PCR Amplification
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 278
EP  - 283
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.039
DO  - 10.2991/absr.k.220406.039
ID  - Sianipar2022
ER  -