The Effects of High-Fat Diet and CCl4 Administration on Liver Function and Lipid Profile in Non-Alcoholic Fatty Liver Disease Rat Model
- DOI
- 10.2991/absr.k.220406.075How to use a DOI?
- Keywords
- Animal model; NAFLD; liver function; lipid profile
- Abstract
Non-alcoholic fatty liver disease (NAFLD) is often encountered in the field of hepatology. The disease has a broad spectrum ranging from non-inflammatory fat-accumulating macrovesicles (simple steatosis) and develops to fibrous and cirrhosis. The rate of NAFLD is 15-30% in western countries and around 5-18% in Asia. This prevalence continues to grow worldwide following the trends in unhealthy lifestyles and diets. A high-fat diet (HFD) can cause fat accumulation in hepatocytes and adipocytes. NAFLD is predicted to be the main cause of morbidity and mortality associated with liver disease. Therefore, it is necessary to conduct studies that can provide solutions to this problem, either using experimental animal models that reflect the pathogenesis of NAFLD or through clinical studies in humans. NAFLD animal models can be induced in several ways, such as through HFD treatment and the use of carbon tetrachloride (CCl4). This study was focused to figure out the influence of combined HFD intervention and CCL4 administration on liver function (aspartate aminotransferase/AST and alanine aminotransferase/ALT levels) and lipid profile (cholesterol and triglycerides levels) as the metabolic parameters in the NAFLD rat model. Twenty-eight Sprague Dawley rats (body weight between 160-210 grams, 8-10 weeks old,) were split within three groups, namely (I) control group, rats were given a standard food (RatBio®); (II) the HFD group, rats were given a high-fat diet (self-made pellets with a composition of 30% fat, 11% protein, 9% fiber, and 14% water) intervention; and (III) the HFDCCl4 group, rats were given HFD intervention and administering intraperitoneal CCl4 (0.5 mg/ml/kg body weight two times a week). Both diet and chemical interventions were carried out for 12 weeks. Rats were weighed every week. Blood samples were taken to measure the liver function parameters (AST and ALT levels) and the lipid profiles (cholesterol and triglycerides levels) pre- (week 0) and post- intervention (week 12). All groups displayed an increase in body weight after the intervention (p=0.000). However, the average weight gain between the three groups did not differ (166.88 + 33.87 g, 188.44 + 30.39 g, and 173.30 + 30.75 g, respectively). The pre-intervention and post-intervention AST and ALT levels in all groups were still within normal limits and did not show significant mean changes (p=0.120 and p=0.811, respectively). The pre-and post-intervention lipid profile (cholesterol and triglyceride levels) in all groups showed significant differences in means (p=0.023 and p=0.036, respectively). From the posthoc test, it was found that the lipid profile of the HFD-CCl4 group was different from the other groups. In conclusion, a NAFLD modeling with a combination of HFD intervention (30% fat content) and CCl4 administration for 12 weeks gave better results than NAFLD modeling only with HFD intervention.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Marwan Rosada AU - Widya Wasityastuti AU - Yanasta Yudo Pratama AU - Ken Siwi AU - Dewiyani Indah Widasari AU - Tutik Sri Wahyuni PY - 2022 DA - 2022/05/02 TI - The Effects of High-Fat Diet and CCl₄ Administration on Liver Function and Lipid Profile in Non-Alcoholic Fatty Liver Disease Rat Model BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 533 EP - 539 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.075 DO - 10.2991/absr.k.220406.075 ID - Rosada2022 ER -