Comparison of Cycle Threshold Value of The Orf 1 ab SARS-CoV-2 Gene from Three Different PCR Reagents
- DOI
- 10.2991/absr.k.220406.090How to use a DOI?
- Keywords
- CT value; Orf 1 ab. Reagents; RT- PCR; SARS-CoV-2
- Abstract
The availability of PCR reagents is an obstacle that still needs to be resolved in the early days of the SARS-CoV-2 pandemic. During the early days of the pandemics, identical PCR reagents supply in the laboratory could not be guaranteed. The laboratory needs to investigate the reliability of the different reagents used for examination, to make sure that the results of the examination are valuable to patient management. This study aims to compare the Cycle Threshold (CT) value of the Orf 1 ab gene from three SARS-CoV-2 PCR reagents used for diagnosis, monitoring of the patient condition, and progress of therapy. The research sample was taken from nasopharyngeal and oropharyngeal swabs of individuals who were in close contact with confirmed SARS-CoV-2 patients. The reagents compared was originated from manufacturers in China and from South Korea. All of the examinations were performed with reverse transcriptase real-time PCR. The study was designed cross-sectionally. Comparative analysis for paired data was performed using Friedman’s test and Wilcoxon’s Post Hoc analysis. The results showed that the median CT value of the Orf 1 ab gene examined with reagents 1, 2, and 3 was 25,96; 24,87, and 28,39. The results of the analysis showed that there was a difference in the CT value of the Orf1 ab gene between the three PCR reagents (p < 0.05). The results showed that if CT value is to be used for diagnosis and monitoring of each patient’s condition then the reagents used in the PCR examination cannot be replaced interchangeably.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Anita L. Susanti AU - Fusvita Merdekawati AU - Rohayati Rohayati PY - 2022 DA - 2022/05/02 TI - Comparison of Cycle Threshold Value of The Orf 1 ab SARS-CoV-2 Gene from Three Different PCR Reagents BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 629 EP - 633 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.090 DO - 10.2991/absr.k.220406.090 ID - Susanti2022 ER -