LC50 and Effect of Sublethal Concentration of K2Cr2O7 on Different Developmental Stages of Osteochilus vittatus
- DOI
- 10.2991/absr.k.220406.042How to use a DOI?
- Keywords
- Chromium; Embryo; Larvae; LC50; Osteochilus vittatus
- Abstract
Chromium in form of hexavalent had been reported to be genotoxic and carcinogenic, affects some physiological, and reproductive features. The ability of aquatic animals including fish to tolerate chromium may vary according to different developmental stages. Therefore, this research was conducted to (1) determine the LC50 of K2Cr2O7 for embryo, larvae, and juvenile of Osteochilus vittatus, and (2) evaluate the chronic exposure of sublethal concentration of K2Cr2O7 in embryo and juvenile. The LC50 96h was determined using Probit analysis. Two experimental studies were conducted using a completely randomized design. In experiment-1: 5 groups of 2-cell stage embryos were exposed to, 0, 20, 40, 60, and 80 ppm of K2Cr2O7, 8 units replicate each containing 200 embryos were provided, the parameters were hatching rate (HR) and survival rate (SR) of 4dph larvae. In experiment-2: three developmental stages i.e. 30dph, 60 dph, and 90 dph juveniles were exposed to 0 ppm, 2.5 ppm, and 5.00ppm of K2Cr2O7, for 30 days; the observed parameters were total body length, body weight, and SR. The results showed that LC50 for embryos was 100ppm, for larvae was 14.37ppm and for juveniles was 8.1ppm. The results of experiment-1 showed that the hatching rate of embryos treated with 0, 20, 40, 60, and 80 ppm of K2Cr2O7 were 90.61±2.52%, 81.13±0.70%, 87,21±4.62%, 76.30±3.20%, and 77.30±4.12% respectively. The survival rate of 4 dph larvae of the treated embryos were 100%, 99.66±0.29%, 98.71±0.84%, 98.31±1.79, and 94.61±1.75 respectively, The results of experiment-2 showed that 30dph juvenile treated with 0ppm, 2.5ppm, and 5.00ppm of K2Cr2O7 have a body length of 14.67±1.52mm, 12.33±5.51mm and 10.00±0.81 respectively; the treated 60dph juvenile have a body length of 26.75±3.88mm, 20.25±3.84mm, 20.62±3.85mm respectively; the treated 90dph juvenile have body length of 37.00±1.7mm, 35.00±2.16mm, and 33.33±1.52mm respectively. The body weights of juveniles exposed to K2Cr2O7 were significantly lower (p<0.1) compare to the control group. The SR of the juveniles exposed to K2Cr2O7 was significantly lower (p<0.01) compared to the control group. Based on all parameters it can be concluded that the LC50 of K2Cr2O7 in O. vittatus was varied according to its developmental stages and its effects was detrimental. Attention needs to be taken to regulating the release of effluent-containing chromium to the environment.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Gratiana E. Wijayanti AU - Sharon Hillary AU - Ani Septiani AU - Anisa Aulia AU - Anastasia E. Sintanora PY - 2022 DA - 2022/05/02 TI - LC50 and Effect of Sublethal Concentration of K2Cr2O7 on Different Developmental Stages of Osteochilus vittatus BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 297 EP - 302 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.042 DO - 10.2991/absr.k.220406.042 ID - Wijayanti2022 ER -