In Vitro Anthelmintic Activity of Limonia acidissima, L. Leaves Aqueous Extract on Haemonchus contortus (Rudolphi, 1803)

DOI

10.2991/absr.k.220406.011How to use a DOI?

Keywords

Anthelmintic; Haemonchus contortus; In Vitro; Limonia acidissima

Abstract

Mortality, loss of production, slowed growth, poor weight gain, and even death are all common economic losses caused by haemonchosis. Due to the emergence of anthelmintic resistance and the expensive expense of chemical anthelmintic treatments, medicinal plants have been investigated as potential anthelmintics. This study aimed to examine the in vitro anthelmintic activity of Limonia acidissima leaves aqueous extract on Haemonchus contortus. Phytochemical analysis of L. acidissima was conducted to measure some secondary metabolites. The efficacy of six extract concentrations (2.5, 5, 7.5, 10, 12.5, and 15 %) was tested through an adult worm motility test while ivermectin 0.1% was positive control and NaCl 0.62% was a negative control. Histology preparation was conducted using the paraffin method and hematoxylin-eosin staining. The secondary metabolites measurement show that tannin is 1.83 %b/v, saponin is 0.23 %b/v, and flavonoid is 0.85 %b/v. These bioactive compounds are believed to have a role in causing the death of worms. The results of adult worm motility revealed that the highest mean inhibition of adult worm motility is at a concentration of 15% and the lowest one is at a concentration of 0.25%. While ivermectin 0,1% showed inhibited motility after 2 hours incubation and in contrast NaCl 0,62 % showed no effect on motility test. All concentrations of L. acidissima aqueous extract showed significantly inhibited the motility of adult worms at a 1% level significance. Duncan’s post hoc test showed that L. acidissima aqueous extract had slow onset of activity as compared to ivermectin 0.1%. Each concentration caused damage to the cuticle and muscular layer of H. contortus. The increase in the level of concentration is directly proportional to the increase in damage to the integumentary structure. The overall findings of this study have shown that L. acidissima leaves aqueous extract has an anthelmintic activity and further evaluation of these plants should be carried out.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Muh. Andhi Hardianto
AU  - Slamet Widiyanto
PY  - 2022
DA  - 2022/05/02
TI  - In Vitro Anthelmintic Activity of Limonia acidissima, L. Leaves Aqueous Extract on Haemonchus contortus (Rudolphi, 1803)
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 73
EP  - 77
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.011
DO  - 10.2991/absr.k.220406.011
ID  - Hardianto2022
ER  -