Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Genetic Variations and Phenetic Relationships of Hairy Bittercress (Cardamine hirsuta L.) Accession in Java based on Inter-Simple Sequence Repeat

Authors
Tania Agnesa1, Purnomo Purnomo2, Budi Setiadi Daryono3, *
1Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
2Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3Laboratory of Plant Systematics, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
*Corresponding author. Email: bs_daryono@mail.ugm.ac.id
Corresponding Author
Budi Setiadi Daryono
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.037How to use a DOI?
Keywords
Cardamine hirsuta L; Genotypic variation; ISSR; Phenetic analysis
Abstract

Cardamine hirsuta from the Family Brassicaceae is a potential plant that can be used as an object of comparative study with Arabidopsis thaliana (L.) Heynh. Plant comparative studies involving closely related species with similar traits are used to investigate the genetic pathways underlying morphological diversity within and between species. This comparative study approach is based on the natural variation of C. hirsuta. Genetic variation can be found at the molecular level, resulting in a more stable classification. Inter-Simple Sequence Repeat (ISSR) markers are frequently used to study the genetic variation of closely related species. The purpose of this study was to examine genetic variation using ISSR markers to determine variations and phenetic relationships of C. hirsuta accessions in Java (Indonesia). Molecular data were numerically analyzed with seven ISSR markers using the DNA fingerprinting technique, including polymorphism percentage and Polymorphic Information Content (PIC) value. The dendrogram construction uses the Jaccard coefficient with the UPGMA method, and the PIC uses the dominant marker equation. The results showed that the ISSR marker effectively estimated the variation of genetic accession of C. hirsuta, which was characterized by the degree of polymorphism formed. The seven ISSR markers formed 54 bands with an average polymorphism proportion of 58.70% and an average PIC value of 0.33. In the dendrogram based on molecular characters, 4 sub-clusters were formed at 92% similarity based on location, namely Cibodas (West Java), Kaliurang (Yogyakarta), Dieng Plateau (Central Java), and Senduro (East Java).

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
10.2991/absr.k.220406.037
ISSN
2468-5747
DOI
10.2991/absr.k.220406.037How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Tania Agnesa
AU  - Purnomo Purnomo
AU  - Budi Setiadi Daryono
PY  - 2022
DA  - 2022/05/02
TI  - Genetic Variations and Phenetic Relationships of Hairy Bittercress (Cardamine hirsuta L.) Accession in Java based on Inter-Simple Sequence Repeat
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 261
EP  - 267
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.037
DO  - 10.2991/absr.k.220406.037
ID  - Agnesa2022
ER  -