Genetic Variations and Phenetic Relationships of Hairy Bittercress (Cardamine hirsuta L.) Accession in Java based on Inter-Simple Sequence Repeat
- DOI
- 10.2991/absr.k.220406.037How to use a DOI?
- Keywords
- Cardamine hirsuta L; Genotypic variation; ISSR; Phenetic analysis
- Abstract
Cardamine hirsuta from the Family Brassicaceae is a potential plant that can be used as an object of comparative study with Arabidopsis thaliana (L.) Heynh. Plant comparative studies involving closely related species with similar traits are used to investigate the genetic pathways underlying morphological diversity within and between species. This comparative study approach is based on the natural variation of C. hirsuta. Genetic variation can be found at the molecular level, resulting in a more stable classification. Inter-Simple Sequence Repeat (ISSR) markers are frequently used to study the genetic variation of closely related species. The purpose of this study was to examine genetic variation using ISSR markers to determine variations and phenetic relationships of C. hirsuta accessions in Java (Indonesia). Molecular data were numerically analyzed with seven ISSR markers using the DNA fingerprinting technique, including polymorphism percentage and Polymorphic Information Content (PIC) value. The dendrogram construction uses the Jaccard coefficient with the UPGMA method, and the PIC uses the dominant marker equation. The results showed that the ISSR marker effectively estimated the variation of genetic accession of C. hirsuta, which was characterized by the degree of polymorphism formed. The seven ISSR markers formed 54 bands with an average polymorphism proportion of 58.70% and an average PIC value of 0.33. In the dendrogram based on molecular characters, 4 sub-clusters were formed at 92% similarity based on location, namely Cibodas (West Java), Kaliurang (Yogyakarta), Dieng Plateau (Central Java), and Senduro (East Java).
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Tania Agnesa AU - Purnomo Purnomo AU - Budi Setiadi Daryono PY - 2022 DA - 2022/05/02 TI - Genetic Variations and Phenetic Relationships of Hairy Bittercress (Cardamine hirsuta L.) Accession in Java based on Inter-Simple Sequence Repeat BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 261 EP - 267 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.037 DO - 10.2991/absr.k.220406.037 ID - Agnesa2022 ER -