Molecular Comparison between Two Similar Asteraceae Species (Synedrella nodiflora (L.) Gaertn. and Eleutheranthera ruderalis (Swartz) Sch._Bip) by the Use of trnL(UAA) - trnF(GAA) Intergenic Spacer

DOI

10.2991/absr.k.220406.008How to use a DOI?

Keywords

Eleutheranthera ruderalis (Swartz) Sch.-Bip; Synedrella nodiflora (L.) Gaertn; trnL (UAA) - trnF (GAA) IGS

Abstract

Synedrella nodiflora (L.) Gaertn and Eleutheranthera ruderalis (Swartz) Sch.-Bip. are two Asteraceae species showing very similar morphological appearances. Both are broad-leaf weed species in some crops throughout many tropical areas. Nevertheless, the individual species can be potentially utilized for human life, in which better knowledge on their phenotypical and molecular characters should be available. This study aims to provide scientific information in distinguishing the two identical species using a molecular marker, i.e. trnL(UAA) – trnF(GAA) intergenic spacer (IGS), which has been frequently used to identify genetic differences at a low levels of plant taxa. Plant samples were collected randomly from several Banyumas Regency, Central Java, Indonesia. Genomic DNAs were extracted using a CTAB method, followed by PCR amplification of the marker employing a pair of universal primers, e B49873 as a forward primer and f A50272 as a reverse primer. Sequencing of the amplicons was carried out using the automated Sanger method with labeled terminators. The results showed that trnL (UAA) - trnF (GAA) IGS in S. nodiflora and E. ruderalis were 312 bp and 317 bp in length, respectively. Sequence alignment between trnL (UAA) - trnF(GAA) IGS of both species showed several indels and base substitutions. Despite no direct correlation between these genetic differences and some observed phenotypic differences, this finding provides molecular data distinguishing both species, potentially used for DNA barcoding.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Murni Dwiati
AU  - Indrawati Indrawati
AU  - Agus Hery Susanto
PY  - 2022
DA  - 2022/05/02
TI  - Molecular Comparison between Two Similar Asteraceae Species (Synedrella nodiflora (L.) Gaertn. and Eleutheranthera ruderalis (Swartz) Sch._Bip) by the Use of trnL(UAA) - trnF(GAA) Intergenic Spacer
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 53
EP  - 58
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.008
DO  - 10.2991/absr.k.220406.008
ID  - Dwiati2022
ER  -