The Importance of Purification and Activity Analysis of the Purified Product of Thrombolytic Protease from Bacillus sp. HSFI-12– A Review

DOI

10.2991/absr.k.220406.052How to use a DOI?

Keywords

Bacillus sp. HSFI-12; Chromatography; Enzyme purification; Thrombosis; Thrombolytic protease

Abstract

Mortality and morbidity of Cardio-vascular diseases (CVDs) have been the major issue in the group of non-communicable diseases worldwide. However, the existing antithrombotic drugs to combat CVD still have many shortcomings in terms of price and safety. Bacillus sp. HSFI-12 had been previously reported as a highly potential producer of thrombolytic protease, in which crude protease has competitive clot lysis or thrombolytic activity. However, the purity and other characteristics of the protease in its purified form have not been reported. The remained task is to ensure that Bacillus sp. HSFI-12, as a source of thrombolytic protease, can be used as an alternative of effective treatment with lower cost to combat thrombosis in cardiovascular diseases. This review aims to provide a highlight the most suitable purification methods to be applied to the protease dialysate of Bacillus sp. HSFI-12 and recommending appropriate assays following the recommended purification methods. Results of this review showed that both ion exchange and gel filtration chromatography (IEC and GFC) methods are suggested to be used in the purification step as a continuation of the dialysis process of HSFI-12 protease previously concentrated using ammonium sulfate. In addition, the results of the review found that the most common assays conducted on purified protease developed as antithrombotic agents are anticoagulation and antiplatelet assays. In conclusion, the purification and activity analysis of the purified product of thrombolytic protease from Bacillus sp. HSFI-12 using chromatographic methods is important to do. This review recommended that aside from anticoagulant and antiplatelet assays, thrombolytic activity tests on HSFI-12 protease dialysate are also important to do to support the protease’s characteristics an antithrombotic agent.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Nurul Islamiyah
AU  - Stalis Norma Ethica
AU  - Muhammad Ardi Afriansyah
AU  - Ana Hidayati Mukaromah
AU  - Dewi Seswita Zilda
PY  - 2022
DA  - 2022/05/02
TI  - The Importance of Purification and Activity Analysis of the Purified Product of Thrombolytic Protease from Bacillus sp. HSFI-12– A Review
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 365
EP  - 372
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.052
DO  - 10.2991/absr.k.220406.052
ID  - Islamiyah2022
ER  -