Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)

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TLC-Bioautographic and LC-MS/MS Detection of Antimicrobial Compounds from Four Semipolar Extracts of Cladonia Species

Authors
Friardi Ismed1, 2, *, Widi Novella Desti1, Nurwahidatul Arifa1, Rustini Rustini1, Deddi Prima Putra1, 2
1Faculty of Pharmacy, Andalas University, Padang 25163, Indonesia
2Laboratory of Biota Sumatera, Andalas University, Padang 25163, Indonesia
*Corresponding author. Email: friardi@phar.unand.ac.id
Corresponding Author
Friardi Ismed
Available Online 17 November 2021.
DOI
10.2991/ahsr.k.211105.008How to use a DOI?
Keywords
Cladonia; depside; depsidone; TLC-bioautography; LC-MS/MS
Abstract

To continue the study of Sumatran lichen, six species of the genus Cladonia were screened for phytochemicals and their antibacterial bioactivity. The procedure included sequential extraction (semi polar solvent), antibacterial activity tests using the agar diffusion method, and microdilution to determine the minimum inhibitory concentration (MIC) value of each extract. The study also involved the thin layer chromatography (TLC)-bioautography method and liquid chromatography-mass spectrometry (LC-MS/MS) analysis (in positive mode), which aimed to provide information about the composition and activity of individual components of the extracts that have an antibacterial effect. The test results indicated that all studied Cladonia lichen semipolar extracts had an antibacterial effect, while five of them had very strong bacterial growth inhibition activity (MIC range values 2.5 to 10 mg/mL). Additionally, the identities of compounds found in lichen with broad inhibition, which include the depside and depsidone groups, were confirmed by LC-MS/MS. Based on existing test data, C. scabriuscula, C. crispata, C. rappii, and C. macilenta are potential antibacterial candidates.

Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
Series
Advances in Health Sciences Research
Publication Date
17 November 2021
ISBN
10.2991/ahsr.k.211105.008
ISSN
2468-5739
DOI
10.2991/ahsr.k.211105.008How to use a DOI?
Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Friardi Ismed
AU  - Widi Novella Desti
AU  - Nurwahidatul Arifa
AU  - Rustini Rustini
AU  - Deddi Prima Putra
PY  - 2021
DA  - 2021/11/17
TI  - TLC-Bioautographic and LC-MS/MS Detection of Antimicrobial Compounds from Four Semipolar Extracts of Cladonia Species
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 49
EP  - 59
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.008
DO  - 10.2991/ahsr.k.211105.008
ID  - Ismed2021
ER  -