Synthesis and characterization of CD44 targeted nanoparticle PEI/pDNA/HA-mPEG for gene delivery
- DOI
- 10.2991/icmia-16.2016.16How to use a DOI?
- Keywords
- PEI/DNA/HA-mPEG nanoparticle, targeted, gene delivery
- Abstract
In order to prepare targeted nanocarrier to gene delivery, a novel nanoparticle was designed to condense pDNA. Hyaluronic acid was regarded as the targeted bio-materials because the HA–receptor-mediated CD44 receptors existed in many issues which were interacted with tumor membrane. The preparation procedure was determined by N/COOH and condensing pDNA capability was measured with agarose gel electrophoresis assay. The HA-mPEG polymer was characterized by FI-IR and PEI/pDNA/HA-mPEG nanoparticle was characterized by Nano Zetasizer. The obtained results was showed that the HA-mPEG was successfully synthesized and the ternary complexes presented relatively uniform particle distribution with 200-450 nm, the zeta potential was increased with the increasing content of PEI. The PEI/DNA/HA-mPEG nanoparticle showed the potential in gene delivery.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Tiantian Wang AU - Mingxing Liu AU - Honghao Sun PY - 2016/11 DA - 2016/11 TI - Synthesis and characterization of CD44 targeted nanoparticle PEI/pDNA/HA-mPEG for gene delivery BT - Proceedings of the 2016 5th International Conference on Measurement, Instrumentation and Automation (ICMIA 2016) PB - Atlantis Press SP - 85 EP - 90 SN - 1951-6851 UR - https://doi.org/10.2991/icmia-16.2016.16 DO - 10.2991/icmia-16.2016.16 ID - Wang2016/11 ER -