In Silico PCR Study Amplifying Mitochondrial ND5 Gene to Detect Food Adulteration

DOI

10.2991/978-94-6463-166-1_64How to use a DOI?

Keywords

in silico PCR; food adulteration; NADH dehydrogenase subnunit 5 (ND5); pork

Abstract

The design of the primers affects the PCR's specificity. A mitochondrial gene called Cyt-b is frequently used in PCR to detect the presence of pork, but because the sequence is nearly identical to that of bovine Cyt-b, false positive results frequently occur in lab tests. Therefore, the purpose of this study is to create primers that amplify NADH dehydrogenase subunit 5 (ND5), another mitochondrial gene, and to carry out in silico PCR experiments utilizing bioinformatics tools. Using Geneious Prime bioinformatic software, the mitochondrial ND5 sequences of Sus scrofa and Bos taurus were pairwise matched after being retrieved from NCBI. For both pigs and cows, primers were created using a species-specific sequence. Using Primer BLAST tools in NCBI, the specificity of the primers was examined. The outcome of this research was two sets of primers that amplified a 467 bp fragment of ND5 that was unique to pork and 206 bp fragment of ND5 specific to bovine.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Moralita Chatri
AU  - Afifatul Achyar
AU  - Dwi Hilda Putri
AU  - Dezi Handayani
PY  - 2023
DA  - 2023/05/22
TI  - In Silico PCR Study Amplifying Mitochondrial ND5 Gene to Detect Food Adulteration
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 538
EP  - 543
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_64
DO  - 10.2991/978-94-6463-166-1_64
ID  - Chatri2023
ER  -