Stability of Production of Active Antibiotic Compounds by Andalas Endophite Bacteria (Morus Macroura Miq.) at Several Subculture Frequency

DOI

10.2991/978-94-6463-166-1_61How to use a DOI?

Keywords

Andalas endophytic bacteria; subculture (serial passage); stability

Abstract

To maintain the viability of bacteria during the development and utilization process, it is necessary to rejuvenate the bacteria through a subculture process. It is interesting to know whether the activity of the antibiotic compounds and the genetic stability of Andalas endophytic bacteria will remain the same when the bacteria are subcultured repeatedly on the growth medium. The purpose of this study was to determine the stability of production of antibiotic active compounds and genetic stability by Andalas endophytic bacteria at several subculture frequencies. The subculture of Andalas endophytic bacteria in this study was carried out on B.J.T.A.2.1 isolates, starting from the 3rd passage (P.3) to the 25th passage (P.25). Subcultures were performed on triple NA slanted agar medium. Stability of production of active antibiotic compounds was carried out using the diffusion method with the point inoculation technique. The genetic stability test of Andalas endophytic bacteria was carried out by comparing the isolate’s 16S rRNA sequence (P.3) with (P.25). The results showed that the stability of the production of antibiotic active compounds decreased after repeated subcultures in the 7th passage. The results of genetic stability research by Andalas endophytic bacteria isolate B.J.T.A.2.1 at several subculture frequencies found 3 nucleotide variations of the 16S rRNA gene between the two Andalas endophytic bacteria isolates.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Dwi Hilda Putri
AU  - Iffa Sakina Haq
AU  - Dezi Handayani
AU  - Violita
AU  - Nurhasnah
AU  - Irdawati
PY  - 2023
DA  - 2023/05/22
TI  - Stability of Production of Active Antibiotic Compounds by Andalas Endophite Bacteria (Morus Macroura Miq.) at Several Subculture Frequency
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 512
EP  - 520
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_61
DO  - 10.2991/978-94-6463-166-1_61
ID  - Putri2023
ER  -