Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis

DOI

10.2991/978-94-6463-166-1_8How to use a DOI?

Keywords

DNA Isolation; PCR; ND5; Phenol-Chloroform

Abstract

About 85% of Indonesia’s population is Moslem. Every Moslem is ordered to eat halal and good food. PCR is the most widely used molecular biology technique for authentication tests, through target DNA analysis, including the detection of pork-derived DNA in food products. DNA isolation is an important step that determines the success of PCR, especially for food samples that have gone through various cooking processes at high temperatures, thus affecting the quantity and quality of DNA isolation results. The phenol-chloroform-based DNA isolation method is a method that’s considered capable of producing high-quality DNA although the process is slightly longer than using commercial kits. This study aims to obtain optimum DNA isolation results using the phenol-chloroform method in meat-based food samples. DNA quality was tested pork-specific ND5 gene PCR Isolation was carried out on pork and meatball samples which were weighed as much as 50 mg and 100 mg. The results showed DNA isolation with a sample weight of 50 mg was better than a sample weight of 100 mg. The DNA concentration at the sample weight of 50 mg and 100 mg were 423.35 ng/µl and 7472.9 ng/µl. DNA purity at the sample of 50 mg and 100 mg were 1.7 and 1.0.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Indah Mardhotillah
AU  - Afifatul Achyar
AU  - Moralita Chatri
AU  - Dwi Hilda Putri
AU  - Yuni Ahda
PY  - 2023
DA  - 2023/05/22
TI  - Optimization of DNA Isolation on Meat-Based Food Samples Using Phenol-Chloroform Method for the Development of Halal Detection Using In-House Method Analysis
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 51
EP  - 58
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_8
DO  - 10.2991/978-94-6463-166-1_8
ID  - Mardhotillah2023
ER  -