Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)

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The Combination Effect of Auxin and Cytokinin on Callus Induction of Patchouli (Pogostemon Cablin Benth.) from Leaf Explants

Authors
Rut Normasari1, 2, *, Estri Laras Arumingtyas1, Rurini Retnowati1, Wahyu Widoretno1
1Department of Biology, University of Brawijaya, Malang, Indonesia
2Department of Agriculture, Klabat University, North Minahasa, Indonesia
*Corresponding author. Email: rutnormasari@student.ub.ac.id
Corresponding Author
Rut Normasari
Available Online 22 May 2023.
DOI
10.2991/978-94-6463-166-1_66How to use a DOI?
Keywords
BA; callus induction; kinetin; NAA; Pogostemon cablin Benth
Abstract

Patchouli, also known as Pogostemon cablin Benth., is a plant that yields patchouli oil, a crucial essential oil used in the perfume industry. Stem cuttings are the conventional method of vegetative propagation for patchouli plants. Tissue culture techniques offer alternative approaches for vegetative propagation of better seeds. In vitro approaches for mass multiplication and plant breeding demand a callus culture that has been developed. The purpose of this study was to assess how leaf explants responded to callus formation in vitro caused by a combination of auxin and cytokinins. Leaf explants were cultivated on Murashige and Skoog medium for four weeks with the addition of NAA (0.5, 1, 2, and 3 mg/L) and BA/kinetin (0.5 mg/L). In the second week, the explants began to develop calluses; these calluses were friable, yellowish-green, and yellowish-white. Without NAA, medium was unable to produce callus. Callus development was controlled by the interaction of auxin and cytokinin. The NAA content was correlated with an increase in callus biomass. In comparison to the combination of BA and NAA, the combination of kinetin and NAA produced a larger callus fresh weight and percentage of callus formation. On MS medium enriched with 3 mg/L NAA + 0.5 mg/L kinetin, the maximum callusing was attained.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 May 2023
ISBN
10.2991/978-94-6463-166-1_66
ISSN
2468-5747
DOI
10.2991/978-94-6463-166-1_66How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Rut Normasari
AU  - Estri Laras Arumingtyas
AU  - Rurini Retnowati
AU  - Wahyu Widoretno
PY  - 2023
DA  - 2023/05/22
TI  - The Combination Effect of Auxin and Cytokinin on Callus Induction of Patchouli (Pogostemon Cablin Benth.) from Leaf Explants
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 551
EP  - 557
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_66
DO  - 10.2991/978-94-6463-166-1_66
ID  - Normasari2023
ER  -