Proceedings of the 6th International Conference of Health Polytechnic Surabaya (ICoHPS 2023)

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The Differences Among the Quality of Pleural Fluid Cytological Smops from Alcohol Fixation, Hair Spray, and NAFS with Papanicolaou Staining

Authors
Acivrida Mega Charisma1, *, Amanda Ramadani1, Gery Pacsi Valery1, Yohanes Ardian Kapri Negara1, Farida Anwari1, Intan Febiola Arianing1
1Program Studi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Anwar Medika, Sidoarjo, Indonesia
*Corresponding author. Email: acie.vrida@uam.ac.id
Corresponding Author
Acivrida Mega Charisma
Available Online 23 December 2023.
DOI
10.2991/978-94-6463-324-5_11How to use a DOI?
Keywords
Cytology; Fixative Solution; Pleural Fluid
Abstract

For the manufacture of cytological preparations, the fixation stage was previously carried out to prevent denaturation, prevent cytolysis and ensure. This study aimed to determine the quality of the picture of cytology preparations fixed by several solutions. This research method was carried out using an experimental laboratory method with a posttest only control group design approach to find out the description of cytology preparations that were given alcohol fixative solution, NAFS, and hair spray. The cytology sample used for preparation was pleural fluid. The data obtained then be processed using the Kruskall-Wallis test SPSS. The results of this study were good image quality from hair spray fixation as much as 20%; NAFS fixation was 6.66%; 70% alcohol fixation was 26.6%; and 96% alcohol fixation was 46.6%. As the conclusion of this study, there was a significant difference in the quality of the picture of the cytology preparations in each fixative solution and 96% alcohol fixative solution resulting in a good picture quality of the cytology preparations.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 6th International Conference of Health Polytechnic Surabaya (ICoHPS 2023)
Series
Advances in Health Sciences Research
Publication Date
23 December 2023
ISBN
10.2991/978-94-6463-324-5_11
ISSN
2468-5739
DOI
10.2991/978-94-6463-324-5_11How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Acivrida Mega Charisma
AU  - Amanda Ramadani
AU  - Gery Pacsi Valery
AU  - Yohanes Ardian Kapri Negara
AU  - Farida Anwari
AU  - Intan Febiola Arianing
PY  - 2023
DA  - 2023/12/23
TI  - The Differences Among the Quality of Pleural Fluid Cytological Smops from Alcohol Fixation, Hair Spray, and NAFS with Papanicolaou Staining
BT  - Proceedings of the 6th International Conference of Health Polytechnic Surabaya (ICoHPS 2023)
PB  - Atlantis Press
SP  - 96
EP  - 103
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-324-5_11
DO  - 10.2991/978-94-6463-324-5_11
ID  - Charisma2023
ER  -