Antifungal Activity of Buni Fruit (Antidesma Bunius L.) Against Malassezia Furfur: In Vitro Study with Disc Diffusion Method

DOI

10.2991/978-94-6463-070-1_2How to use a DOI?

Keywords

Antidesma bunius; antifungal; ketoconazole; Disc Diffusion Method

Abstract

Pityriasis versicolor, also known as tinea versicolor, is the most common fungal skin disease found in Indonesia and is mostly caused by the Malassezia furfur. In herbal medicine, one of the natural products is Buni fruit which has active compounds such as tannins, saponins, flavonoids, phenols, and alkaloids that can be used as antifungals. Using the Disc Diffusion method, this study will investigate the effectiveness of the inhibitory power of buni fruit extract (Antidesma bunius L.) against Malassezia furfur in vitro. This is an experimental investigation employing the Disc Diffusion method in vitro with concentrations of buni fruit extracts of 20%, 40%, and 80% with Dimethyl Sulfoxide (DMSO) 10% as the solvent. The buni fruit used was extracted with 70% ethanol and came from Hasanuddin University’s Laboratory of Microbiology, Faculty of Medicine. Twenty samples of Malassezia furfur were grown in Sabouraud Dextrose Agar (SDA), which were divided into four groups: Ketoconazole 200mg as a positive control group, a concentration group of 20%, 40%, and 80%, and a negative control group. A caliper was used to measure the diameter of the inhibition zone generated around the paper disc of buni fruit extract (Antidesma bunius L.) after 24 h. The average diameter of the extract inhibition zone was 7.7 ± 0.22 mm at 20% concentration, 8.2 ± 0.51 mm at 40% concentration, and 8.98 ± 0.64 mm at 80% concentration. It can be stated that the extract of Buni fruit has an antifungal impact on the growth of Malassezia furfur.

Copyright

© 2022 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Dara Ugi Aras
AU  - Mochammad Hatta
AU  - Suryani Tawali
AU  - Rezki Ainun Jariah
PY  - 2022
DA  - 2022/12/26
TI  - Antifungal Activity of Buni Fruit (Antidesma Bunius L.) Against Malassezia Furfur: In Vitro Study with Disc Diffusion Method
BT  - Proceedings of the International Conference on Sustainable Innovation on Health Sciences and Nursing (ICOSI-HSN 2022)
PB  - Atlantis Press
SP  - 3
EP  - 6
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-070-1_2
DO  - 10.2991/978-94-6463-070-1_2
ID  - Aras2022
ER  -