Proceedings of the International Conference on Science and Technology (ICST 2018)

The Morphological, Anatomical, And Physiological Characteristics of Elephantopus scaber As Explant Source For Tissue Culture

Authors
Yuliani Yuliani, Sari Kusuma Dewi, Fida Rachmadiarti
Corresponding Author
Yuliani Yuliani
Available Online December 2018.
DOI
https://doi.org/10.2991/icst-18.2018.13How to use a DOI?
Keywords
Morphological Characteristics, Anatomy, Physiology, Elephantopus scaber, Explant Source, Tissue Culture
Abstract
The purpose of this study was to describe morphological characteristic (leaf area, plant height, and leaf sheath), to describe anatomical characteristic (trichomes found in leaf sheath, veins, leaf blade and stem, to describe the physiological characteristic from phytochemical tests of secondary metabolites from Elephantopus scaber leaf extract, to determine the correct explant source for tissue culture to avoid contamination and to cultivate good callus. The type of this study was descriptive. The study included morphological measurements of E. scaber sampled from Trawas, Mojokerto (midland 700m-950m above sea level). 20 leaves and leaf sheaths for were sampled for measurement of morphological characteristics. 20 plants were also sampled for height measurement. Morphological data described trichomes on leaf blade, veins, and leaf sheath. Microscopic observations of trichomes on leaf blade, veins, and leaf sheath were conducted to describe anatomical characteristics. Phytochemical content of the methanol extract of E. scaber plant leaves with various methods were conducted to describe physiological characteristics so the optimum explant source of the E. scaber could be determined. The results showed that E. scaber that lived in midlands had characteristics, ie average leaf area = 25.78 cm2, leaf sheath length = 1.86 cm and plant height of 3.66 cm, with needle-shaped non-glandular trichomes, and the leaves contained flavonoids, saponins, phenols and triterpenoids. The best explant source was found on the leaf bone which shows 11 specimens live two weeks after planting.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Science and Technology (ICST 2018)
Series
Atlantis Highlights in Engineering
Publication Date
December 2018
ISBN
978-94-6252-650-1
ISSN
2589-4943
DOI
https://doi.org/10.2991/icst-18.2018.13How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yuliani Yuliani
AU  - Sari Kusuma Dewi
AU  - Fida Rachmadiarti
PY  - 2018/12
DA  - 2018/12
TI  - The Morphological, Anatomical, And Physiological Characteristics of Elephantopus scaber As Explant Source For Tissue Culture
BT  - Proceedings of the International Conference on Science and Technology (ICST 2018)
PB  - Atlantis Press
SP  - 61
EP  - 66
SN  - 2589-4943
UR  - https://doi.org/10.2991/icst-18.2018.13
DO  - https://doi.org/10.2991/icst-18.2018.13
ID  - Yuliani2018/12
ER  -