Proceedings of the International Conference on Science and Technology (ICST 2018)

Encapsulation and Germination of Synthetic Seeds of Chrysanthemum

Authors
Pangesti Nugrahani, Ida Retno Moeljani, Irda Lydiana
Corresponding Author
Pangesti Nugrahani
Available Online December 2018.
DOI
https://doi.org/10.2991/icst-18.2018.27How to use a DOI?
Keywords
Encapsulation, Germination, Synthetic Seed, Chrysanthemum
Abstract
Chrysanthemum is one of the most important Indonesia flowers, that which has always been developed in various plant breeding studies, including the development of synthetic seed encapsulation methods. Encapsulation is a technology on enclosed any meristematic plant tissue in a protective and nutritive matrix under in vitro or in vivo environmental conditions, also after transport and/or storage. The study were aimed at developing a method of encapsulating shoot tip and nodal segments of Chrysanthemum that can be used for plant propagation and as explants for short-term storage. Shoot tip and nodal segments of Chrysanthemum were used as explants for synthetic seed. The Murashige & Skoog (MS) basal medium was prepared with 30 g sucrose and 4% of sodium alginate added with growth regulator (IBA) and Coconut Water (CW). The explants droplets, each containing one shoot tip, were then maintained in 2% CaCl2 solution for polymerization. The seeds that have been formed were rinsed with distilled water sterile 3 times and dried at room temperature. The synthetic seeds were germinated on MS basal medium, without any plant growth regulators. Encapsulated seeds had a higher germination percentage in MS salt solution as the gel matrix supplemented with 150 ml/L coconut water. The viability of synthetic seeds was decrease on 4 weeks storage period, compared to the 1, 2 and 3 week’s storage period
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Volume Title
Proceedings of the International Conference on Science and Technology (ICST 2018)
Series
Atlantis Highlights in Engineering
Publication Date
December 2018
ISBN
978-94-6252-650-1
ISSN
2589-4943
DOI
https://doi.org/10.2991/icst-18.2018.27How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Pangesti Nugrahani
AU  - Ida Retno Moeljani
AU  - Irda Lydiana
PY  - 2018/12
DA  - 2018/12
TI  - Encapsulation and Germination of Synthetic Seeds of Chrysanthemum
BT  - Proceedings of the International Conference on Science and Technology (ICST 2018)
PB  - Atlantis Press
SP  - 126
EP  - 129
SN  - 2589-4943
UR  - https://doi.org/10.2991/icst-18.2018.27
DO  - https://doi.org/10.2991/icst-18.2018.27
ID  - Nugrahani2018/12
ER  -