Encapsulation and Germination of Synthetic Seeds of Chrysanthemum
Pangesti Nugrahani, Ida Retno Moeljani, Irda Lydiana
Available Online December 2018.
- https://doi.org/10.2991/icst-18.2018.27How to use a DOI?
- Encapsulation, Germination, Synthetic Seed, Chrysanthemum
- Chrysanthemum is one of the most important Indonesia flowers, that which has always been developed in various plant breeding studies, including the development of synthetic seed encapsulation methods. Encapsulation is a technology on enclosed any meristematic plant tissue in a protective and nutritive matrix under in vitro or in vivo environmental conditions, also after transport and/or storage. The study were aimed at developing a method of encapsulating shoot tip and nodal segments of Chrysanthemum that can be used for plant propagation and as explants for short-term storage. Shoot tip and nodal segments of Chrysanthemum were used as explants for synthetic seed. The Murashige & Skoog (MS) basal medium was prepared with 30 g sucrose and 4% of sodium alginate added with growth regulator (IBA) and Coconut Water (CW). The explants droplets, each containing one shoot tip, were then maintained in 2% CaCl2 solution for polymerization. The seeds that have been formed were rinsed with distilled water sterile 3 times and dried at room temperature. The synthetic seeds were germinated on MS basal medium, without any plant growth regulators. Encapsulated seeds had a higher germination percentage in MS salt solution as the gel matrix supplemented with 150 ml/L coconut water. The viability of synthetic seeds was decrease on 4 weeks storage period, compared to the 1, 2 and 3 week’s storage period
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Pangesti Nugrahani AU - Ida Retno Moeljani AU - Irda Lydiana PY - 2018/12 DA - 2018/12 TI - Encapsulation and Germination of Synthetic Seeds of Chrysanthemum BT - Proceedings of the International Conference on Science and Technology (ICST 2018) PB - Atlantis Press SP - 126 EP - 129 SN - 2589-4943 UR - https://doi.org/10.2991/icst-18.2018.27 DO - https://doi.org/10.2991/icst-18.2018.27 ID - Nugrahani2018/12 ER -