Proceedings of the International Conference on Science and Technology (ICST 2018)

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Authors
Sutini Sutini, Widiwurjani Widiwurjani, Djoko Agus Purwanto, Wirdhatul Muslihatin
Corresponding Author
Sutini Sutini
Available Online December 2018.
DOI
https://doi.org/10.2991/icst-18.2018.51How to use a DOI?
Keywords
cultured in vitro, callus Camellia sinensis L, polyphenon
Abstract
The purpose of this study was to obtain a polyphenon profile from callus extract obtained from in vitro culture of Camellia sinensis L. Polyphenon is one of the bioactive compounds found in Camellia sinensis L plants which can also be produced through in vitro culture. Polyphenon as a bioactive is often used in various fields including in the fields of industry: manufacturing, food-drinks, health, agriculture, computers. To get a certain amount of polyphenon from in vitro culture, Camellia sinensis L callus was first extracted with water solvent, as well as non-water solvents / organic solvents such as ethyl alcohol. The methods used were: (1) inoculation of leaf explants from Camellia sinensis L in vitro on the media plus growth regulators to form callus, (2) treatment to be able to be reused (3) reap the callus followed by callus weighing and callus observation. (3) extraction and isolation of bioactive polyphenon (4) identification of polyphenon from Camellia sinensis L callus with high-performance liquid chromatography qualitatively and quantitatively. The results obtained are a form of polyphenon chromatogram profile from callus Camellia sinensis L which resembles a standard chromatogram of polyphenon.
Open Access
This is an open access article distributed under the CC BY-NC license.

Download article (PDF)

Volume Title
Proceedings of the International Conference on Science and Technology (ICST 2018)
Series
Atlantis Highlights in Engineering
Publication Date
December 2018
ISBN
978-94-6252-650-1
ISSN
2589-4943
DOI
https://doi.org/10.2991/icst-18.2018.51How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Sutini Sutini
AU  - Widiwurjani Widiwurjani
AU  - Djoko Agus Purwanto
AU  - Wirdhatul Muslihatin
PY  - 2018/12
DA  - 2018/12
TI  - Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol
BT  - Proceedings of the International Conference on Science and Technology (ICST 2018)
PB  - Atlantis Press
SP  - 244
EP  - 248
SN  - 2589-4943
UR  - https://doi.org/10.2991/icst-18.2018.51
DO  - https://doi.org/10.2991/icst-18.2018.51
ID  - Sutini2018/12
ER  -