Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

DOI

10.2991/ahsr.k.200215.021How to use a DOI?

Keywords

anti-inflammatory, edema, COX-2, iNOS

Abstract

Inflammatory is a body defense mechanism due to respond of tissue on undermine influences, either local or the one that go into the body. Inflammatory can cause various physical dysfunctions. Uncaria gambir Roxb, a high catechin content in ethyl acetate fraction, is widely used as wound healing in Indonesian culture, therefore its mechanism of anti-inflammatory action is still unknown. The objective of our study is to investigated the activity of anti-inflammatory ethyl acetate fraction of Uncaria gambir leaves inhibition of edema, COX-2 and iNOS expression using induction method with carrageenan in rats. This study is experimental research which use 25 white Wistar strain rats as animals assay that divided into 5 groups: negative group (water), positive group (sodium diclofenac 50 mg/kg bw), dose I (5 mg/kg bw fraction), II (10 mg/kg bw fraction) and III (20 mg/kg bw fraction) groups. Each of them were given orally 30 min before carrageenan 3% induced. The paw volume was measured every day for seven day after injection carrageenan using plethysmometer. Inhibition COX-2 and iNOS expression were determined by enzyme-linked immunosorbent assay. The results showed that ethyl acetate fraction of Uncaria gambir leaves in all doses had anti-inflammatory effect on white rats through the mechanism of inflammatory inhibition by reducing edema volume. All three fraction doses can also reduce COX-2 and iNOS expression. The fraction had the highest anti-inflammatory potency by the dose of 20 mg/kg body weight. The conclusion that ethyl acetate of three doses had anti-inflammatory activities through a mechanism of edema volume reduction, COX-2 and iNOS expression.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Nanang Yunarto
AU  - Putri Reno Intan
AU  - Arifayu Addiena Kurniatri
AU  - Indah Sulistyowati
AU  - Nurul Aini
PY  - 2020
DA  - 2020/02/22
TI  - Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression
BT  - Proceedings of the 4th International Symposium on Health Research (ISHR 2019)
PB  - Atlantis Press
SP  - 108
EP  - 112
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200215.021
DO  - 10.2991/ahsr.k.200215.021
ID  - Yunarto2020
ER  -