Proceedings of the 4th International Symposium on Health Research (ISHR 2019)

Curcuma zanthorrhiza Extracts Induce G2/M Cell Cycle Arrest and Apoptosis in 4T1 and MCF-7 Human Breast Cancer Cells

Authors
Sari Haryanti, Galuh Ratnawati, Nuning Rahmawati
Corresponding Author
Sari Haryanti
Available Online 22 February 2020.
DOI
10.2991/ahsr.k.200215.079How to use a DOI?
Keywords
Curcuma zanthorrhiza, cell cycle, apoptosis
Abstract

Curcuma zanthorrhiza Roxb., known as Javanese turmeric or temulawak in Indonesia, has been reported to have several biological activities, including anticarcinogenic effects. In this study, we aimed to evaluate the effects of temulawak rhizome extract on the cell viability, cell cycle distribution, apoptosis induction, and antimigratory effect of human breast cancer cell line. The rhizome powdre was macerated using ethanol 96% to obtain dry extract. The cell lines (MCF-7 and 4T1) were subjected to increasing doses of temulawak extract and cell viability was quantified by MTT assay. Cell cycle profile and apoptosis induction were done using flow cytometry assay. In vitro wound healing assay was performed on 4T1 cells to evaluate antimigratory effect. Temulawak extract inhibited the growth of MCF-7 and 4T1 cells with the IC50 value 46 and 37 μg/ml respectively. Temulawak extracts induced cell cycle arrest in G2/M phase and apoptosis in MCF-7 and 4T1 cells. The extract was not show anti-migration effect in wound healing assay. This study confirms the potential cytotoxic effects of temulawak in breast cancer cell line and could be developed as a potential chemopreventive agent.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th International Symposium on Health Research (ISHR 2019)
Series
Advances in Health Sciences Research
Publication Date
22 February 2020
ISBN
10.2991/ahsr.k.200215.079
ISSN
2468-5739
DOI
10.2991/ahsr.k.200215.079How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Sari Haryanti
AU  - Galuh Ratnawati
AU  - Nuning Rahmawati
PY  - 2020
DA  - 2020/02/22
TI  - Curcuma zanthorrhiza Extracts Induce G2/M Cell Cycle Arrest and Apoptosis in 4T1 and MCF-7 Human Breast Cancer Cells
BT  - Proceedings of the 4th International Symposium on Health Research (ISHR 2019)
PB  - Atlantis Press
SP  - 415
EP  - 419
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200215.079
DO  - 10.2991/ahsr.k.200215.079
ID  - Haryanti2020
ER  -