Improving Multidrug-Resistance Tuberculosis Papua’s Management Using Whole Genome Sequencing

DOI

10.2991/ahsr.k.200215.001How to use a DOI?

Keywords

stochastic MDR-TB, tuberculosis, whole genome sequencing, rifampicin

Abstract

One of the challenges in TB control of Papua is increasing number of multidrug-resistance tuberculosis (MDR-TB). The aim of the study is to imply the WGS tools for predicting TB drugs resistance even MDR as well XDR in clinical samples as an initiation model of laboratory improvement for accurate treatment in MDR TB. Twenty Lowenstein Jansen M. tuberculosis cultures from sputum clinical samples were taken as a DNA source for WGS. Samples preparation was perform using Nextera XT Kit by Illumina and read by Mi-Seq whole genome sequencing. Sequencing result was analyze using TB profiler and visualize by UGENE. We found mutations that direct to drugs resistance in 19 samples consist of 14 samples are MDR and 5 samples are non MDR mutation. Among the 4 samples of non MDR, 2 of them previously show mutation of rifampicin resistance in gene expert but non mutation resistance in WGS. Whole Genome Sequencing technology is the advance technology that may apply to predict the resistance of TB drugs even MDR or XDR and distinguish the accurate drugs for patients individually.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yustinus Maladan
AU  - Hana Krismawati
AU  - Antonius Oktavian
AU  - C. S Whinie Lestari
PY  - 2020
DA  - 2020/02/22
TI  - Improving Multidrug-Resistance Tuberculosis Papua’s Management Using Whole Genome Sequencing
BT  - Proceedings of the 4th International Symposium on Health Research (ISHR 2019)
PB  - Atlantis Press
SP  - 1
EP  - 7
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200215.001
DO  - 10.2991/ahsr.k.200215.001
ID  - Maladan2020
ER  -