Induction of Plantlet Regeneration on Shallot (Allium cepa var. Aggregatum)

DOI

10.2991/absr.k.210609.038How to use a DOI?

Keywords

Allium cepa var. Aggregatum, micropropagation, MS medium, NAA, sucrose

Abstract

Micropropagation is an alternative method for producing pathogenic contamination-free shallots seedlings. The aim of this research was to determine the optimum balance of auxin-cytokinin and sucrose concentration in stimulating the plantlet regeneration on shallot. The research was conducted from March to August 2019. In this study, the auxin and cytokinin treatment (1-5 ppm) were used in the format of a randomized complete block design in three replications. The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g.L-1 agar powder and were adjusted at pH 5.8 before autoclaving. The multiple shootlet (10 weeks of cultured) were then subcultured for bulblet formation in MS medium supplemeted with BAP dan succrose (3-9%). The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g L-1 agar powder and were adjusted at pH 5.8 before autoclaving. The results showed that the use of ½ MS media could increase the efficiency of using nutrient solution of tissue culture media. Micro shoot formation can be initiated on ½ MS medium with the addition of 1 ppm BAP (1.36 shoots / explants). This media also produced the best response for the number of micro roots (5.63 roots / explants), and the number of micro leaves (3.91 leaves). However, the difference in composition of nutrient in culture media was not able to accelerate the time of growth of shoots, roots and leaves. Further subculture in ½ strength of MS medium containing of BAP and sucrose 6% increased shoot, root and bulblet formation (13.3 shoots, 41.3 roots and 10.7 bulblets).

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - M. Marlin
AU  - M. Handajani
AU  - Y. Yulian
AU  - R. Rustikawati
AU  - R. Herawati
PY  - 2021
DA  - 2021/06/11
TI  - Induction of Plantlet Regeneration on Shallot (Allium cepa var. Aggregatum)
BT  - Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)
PB  - Atlantis Press
SP  - 239
EP  - 244
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210609.038
DO  - 10.2991/absr.k.210609.038
ID  - Marlin2021
ER  -