Exploration of Fish Gut Associated Actinobacteria for its Anti-Microbial and Anti-Quorum Sensing Properties

DOI

10.2991/absr.k.210609.021How to use a DOI?

Keywords

Actinobacteria, fish gut, Anti-quorum sensing, Antimicrobial activity and phylogenetic analysis

Abstract

The study was undertaken to explore the gut associated actinobacteria from marine fish with specific to antimicrobial and anti-quorum sensing activities. A total of 25 actinobacterial strains were isolated from fish gut samples using starch casein agar and Kuster’s agar medium. About 12 morphologically different strains recovered from Rastrelliger kanagurta (Indian mackerel), Decapodiformes (squid), Lutjanus campechanus (red snapper) and Caranx melampyqus (bluefin trevally) were screened for the antimicrobial activity against Staphylococcus aureus MTCC96, Escherichia coli MTCC739, Candida albicans, and quorum sensing inhibition (QSI) against Chromobacterium violaceum. The actinobacterial strain SQA4 from Decapodiformes (squid) showed both antimicrobial and QSI activity. Strain SQA4, which showed wide range of activity, were selected as the potential strain for further studies. Bioactive metabolites production, strain SQA4 produced bioactive metabolites compound earlier on ISP2 agar when compared to ISP2 broth. Crude antimicrobial from the strain SQA4 was produced by adopting agar surface fermentation and extracted using ethyl acetate. Based on the studied phenotypic characteristics, actinobacterial strain SQA4 was identified as Streptomyces sp. In optimization, it was found that various sugars and nitrogen compounds and minerals were found to influence the antiquorum sensing compound production. BLAST and phylogenetic tree analysis of SQA4 shows 99% similarity to sequence of Streptomyces maritimus. Further studies like purification and structure elucidation of the compound will be done in future. Thus, the findings suggested that the fish-associated actinobacteria is a promising source for potential bioactive compounds for developing novel therapeutic drugs.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - M. Vadivel
AU  - G. Venugopal
AU  - V. Angamuthu
AU  - R. Manikkam
AU  - J. Joseph
AU  - W. Aruni
PY  - 2021
DA  - 2021/06/11
TI  - Exploration of Fish Gut Associated Actinobacteria for its Anti-Microbial and Anti-Quorum Sensing Properties
BT  - Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020)
PB  - Atlantis Press
SP  - 126
EP  - 139
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210609.021
DO  - 10.2991/absr.k.210609.021
ID  - Vadivel2021
ER  -