Antimicrobial Efficacy of Methanobactin against Bacillus Subtilis

DOI

10.2991/meici-15.2015.56How to use a DOI?

Keywords

Methanobactin; Antimicrobial; Bacillus Subtilis; MinimumInhibitory Concentrations

Abstract

Methanobactin is a novel extracellular fluorescent chromopeptide produced by methanotrophys. Its recent characterization has placed it in a new class of compounds, which is now known as chalkophores. They are analogous to iron-binding siderophores. The present study investigated the antimicrobial efficacy of copper-bound methanobactin (Mb-Cu) against Bacillus Subtilis in LB medium. Minimum inhibitory concentrations (MICs) determined that at 24 h for stationary-phase Bacillus Subtilis cultures in liquid LB medium (36°C) via use of a two-fold dilutions method, was 5 mg/mL. Minimum bactericidal concentrations (MBC) for Mb-Cu observed in the plate after 72 h at 36°C was 10 mg/mL. Effect of different pH value (2 to 9) on antibacterial activity of Mb-Cu was measured by bacteriostatic circle method; the results showed that there was a non-liner relationship between the antimicrobial activity of Mb-Cu against Bacillus Subtilis and pH. Methanobactin was active in the range of pH 2 9 and has the best antimicrobial activity at near pH 6.0. Growth curve of Bacillus subtilis results showed that the maximum growth rate of Bacillus Subtilis was reduce and the lag phase was extended for 3 h.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Haili Xing
AU  - Jiaying Xin
PY  - 2015/06
DA  - 2015/06
TI  - Antimicrobial Efficacy of Methanobactin against Bacillus Subtilis
BT  - Proceedings of the 2015 International Conference on Management, Education, Information and Control
PB  - Atlantis Press
SP  - 305
EP  - 309
SN  - 1951-6851
UR  - https://doi.org/10.2991/meici-15.2015.56
DO  - 10.2991/meici-15.2015.56
ID  - Xing2015/06
ER  -