The effects of a growth hormone-releasing hormone antagonist and a gastrin-releasing peptide antagonist on intimal hyperplasia of the carotid artery after balloon injury in a diabetic rat model☆

John C. Moscona; Matthew N. Peters; Andrew V. Schally; Sudesh Srivastav; Patrice Delafontaine; Anand Irimpen

doi:10.1016/j.artres.2017.06.006

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Volume 19, Issue C, September 2017, Pages 56 - 64

The effects of a growth hormone-releasing hormone antagonist and a gastrin-releasing peptide antagonist on intimal hyperplasia of the carotid artery after balloon injury in a diabetic rat model☆

Authors

John C. Moscona^a^{, *}, Matthew N. Peters^b, Andrew V. Schally^c^{, d}^{, e}, Sudesh Srivastav^f, Patrice Delafontaine^g, Anand Irimpen^a^{, h}

^aTulane University Heart & Vascular Institute, New Orleans, LA, USA

^bUniversity of Maryland School of Medicine, Division of Cardiovascular Medicine, Baltimore, MD, USA

^cUniversity of Miami, Department of Medicine and Pathology, Miami, FL, USA

^dDepartment of Endocrine, Polypeptide, and Cancer Institute, Veterans Affairs Medical Center, Miami, FL, USA

^eVeterans Affairs Hospital, New Orleans, LA, USA

^fTulane University School of Public Health and Tropical Medicine, New Orleans, LA, USA

^gUniversity of Missouri-Columbia, School of Medicine, USA

^hSoutheast Louisiana Veterans Healthcare System, New Orleans, LA, USA

^☆

This research was partially presented as an abstract at the American College of Cardiology 2013 Scientific Sessions with publication as a supplement in the Journal of the American College of Cardiology: (J Am Coll Cardiol. 2013; 61 (10_S): doi:10.1016/S0735-1097(1361178)) however it has not been published in any other form and is being considered solely by Artery Research.

^*Corresponding author. 3442 Vincennes Place, New Orleans, 70125, LA, USA. Fax: +1 504 988 9045. E-mail address: jcmoscona@gmail.com (J.C. Moscona).

Corresponding Author

John C. Moscona

Received 19 May 2017, Revised 23 June 2017, Accepted 28 June 2017, Available Online 13 July 2017.

DOI: 10.1016/j.artres.2017.06.006 How to use a DOI?
Keywords: Neointimal hyperplasia; Growth hormone-releasing hormone antagonist; Gastrin-releasing peptide antagonist; Arterial restenosis; Diabetes mellitus
Abstract: Introduction: Arterial restenosis after angioplasty/stenting has hindered coronary artery disease treatment, especially in diabetics. We theorized that gastrin-releasing peptide (GRP) antagonists and growth hormone-releasing hormone (GHRH) antagonists might decrease neointimal hyperplasia and restenosis in diabetic rats after common carotid arterial balloon injury.

Methods: Two separate experiments were conducted to test the effects of a GRP antagonist (RC-3095) and a GHRH antagonist (MZ-4-71) on vascular smooth muscle (VSM) growth. In a preliminary in vitro experiment non-injured human aortic vascular smooth muscle (VSM) proliferation was compared between growth media and control. In a second in vivo experiment, intimal and medial area, intima/media ratio (IM) and percent stenosis were compared between injured carotid arteries in twelve Zucker type II obese rats treated with subcutaneously injected RC-3095, MZ-4-71, or control media.

Results: In the in vitro experiment, decreased VSM cell growth was observed in GRP antagonist (p < 0.05) and GHRH antagonist groups (p < 0.05) compared to the control group. In the in vivo experiment, the GRP antagonist group had a decreased IM ratio (1.63 ± 0.41, p < 0.05) and an increased area of stenosis (98.78% ± 1.48 p = NS) compared to control (2.38 ± 1.09) while the GHRH antagonist group had decreased IM ratio (1.33 ± 0.58 SD, p < 0.05) and percent area of stenosis (78.84% ± 24.97, p < 0.05) compared to control (2.38 ± 1.09).

Conclusions: The significant decrease in both IM ratio and percent area of stenosis in the GHRH antagonist group supports the hypothesis that this peptide may reduce neointimal hyperplasia and restenosis.
Open Access: This is an open access article distributed under the CC BY-NC license.

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Journal: Artery Research
Volume-Issue: 19 - C
Pages: 56 - 64
Publication Date: 2017/07/13
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2017.06.006 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

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ris enw bib

TY  - JOUR
AU  - John C. Moscona
AU  - Matthew N. Peters
AU  - Andrew V. Schally
AU  - Sudesh Srivastav
AU  - Patrice Delafontaine
AU  - Anand Irimpen
PY  - 2017
DA  - 2017/07/13
TI  - The effects of a growth hormone-releasing hormone antagonist and a gastrin-releasing peptide antagonist on intimal hyperplasia of the carotid artery after balloon injury in a diabetic rat model☆
JO  - Artery Research
SP  - 56
EP  - 64
VL  - 19
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.06.006
DO  - 10.1016/j.artres.2017.06.006
ID  - Moscona2017
ER  -

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