Artery Research

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Volume 5, Issue 4, December 2011, Pages 165 - 166

P5.08 NORMALISATION FOR WALL SHEAR STRESS STIMULUS IMPROVES ACCURACY IN THE ASSESSMENT OF GLUCOSE-INDUCED CHANGES IN FLOW-MEDIATED DILATION

Authors
F.C.G. van Bussel, B.C.T. van Bussel, M.T.J. van Bussel, J. Op’t Roodt, A.P.G. Hoeks, C.G. Schalkwijk, K.D. Reesink
Maastricht University Medical Centre, Maastricht, Netherlands
Available Online 29 November 2011.
DOI
10.1016/j.artres.2011.10.065How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Background: In healthy arteries, acute changes in wall shear stress (WSS) elicit a vessel dilation response, through endothelial NO-release and smooth muscle relaxation (flow-mediated dilation, FMD). Metabolic or pharmacologic agents might modulate FMD, but may also interfere with the transient WSS stimulus utilised to assess FMD. We hypothesized that glucose-induced changes in FMD can be detected with greater sensitivity by normalising FMD for the WSS stimulus.

Methods & Results: Twenty-two healthy volunteers (24±6 yrs, BMI 24±7kg/m2) underwent a standard oral glucose tolerance test during which brachial FMD was assessed by simultaneous B-mode/PW-Doppler ultrasound at baseline (twice), at 30 and at 75min post glucose intake. FMD was defined as the peak increase in diameter relative to baseline diameter (ΔDpeak/Dbaseline) and as the response/stimulus ratio: nFMD = FMD/(ΔWSSmean/WSSbaseline). Subjects underwent this protocol twice, resulting in four baseline FMD measurements to estimate intra-class correlation coefficients, which were 0.57 and 0.63 for nFMD and FMD, respectively.

Conclusions: With nFMD, glucose induced changes in flow mediated dilation are better discriminated than by ΔDpeak/Dbaseline. These findings demonstrate the merit of stimulus normalisation in vascular intervention studies.

baseline 30min 75min p-value*
glucose [mmol/I] 4.7 ± 0.3 6.6 ±1.0 5.9 ± 1.3 <0.001
nFMD [a.u.] 0.072 ± 0.056 0.029 ±0.019 0.037 ± 0.035 0.001
FMD [%] 6.3 ±2.7 5.1 ±3.0 5.9 ± 3.4 0.068
*

repeated ANOVA, n=22.

Table 1

Effect of the glucose tolerance test: comparision between nFMD and FMD

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Journal
Artery Research
Volume-Issue
5 - 4
Pages
165 - 166
Publication Date
2011/11/29
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2011.10.065How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

risenwbib
TY  - JOUR
AU  - F.C.G. van Bussel
AU  - B.C.T. van Bussel
AU  - M.T.J. van Bussel
AU  - J. Op’t Roodt
AU  - A.P.G. Hoeks
AU  - C.G. Schalkwijk
AU  - K.D. Reesink
PY  - 2011
DA  - 2011/11/29
TI  - P5.08 NORMALISATION FOR WALL SHEAR STRESS STIMULUS IMPROVES ACCURACY IN THE ASSESSMENT OF GLUCOSE-INDUCED CHANGES IN FLOW-MEDIATED DILATION
JO  - Artery Research
SP  - 165
EP  - 166
VL  - 5
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2011.10.065
DO  - 10.1016/j.artres.2011.10.065
ID  - vanBussel2011
ER  -