Artery Research

Volume 24, Issue C, December 2018, Pages 75 - 75

4.1 PROBING ARTERIAL STIFFNESS AT THE NANO-SCALE USING THE INTERNAL MAMMARY ARTERY AS A NOVEL TARGET

Authors
Riaz Akhtar1, Zhuo Chang2, Maria Lyck Hansen3, Hans Christian Beck3, Lars Melholt Rasmussen3
1University of Liverpool, United Kingdom
2University of Liverpool, Liverpool, United Kingdom
3Odense University Hospital, University of Southern Denmark, Denmark
Available Online 4 December 2018.
DOI
10.1016/j.artres.2018.10.040How to use a DOI?
Abstract

Introduction: Arterial stiffening is associated with structural and biomechanical alterations in the aorta. However, there are still gaps in our understanding as to how the structure and properties of arteries across the vasculature are altered with high PWV.Objective: To determine whether altered ultrastructural and nanomechanical properties are exhibited in the internal mammary artery (IMA) in high PWV patients.

Methods: Human IMA biopsies were obtained from patients with known carotid-femoral PWV. Patients were grouped as low PWV (8.5 ± 0.7 ms−1, n = 8) and high PWV (13.4 ± 3.0 ms−1, n = 9). With Peakforce QNM atomic force microscopy (AFM) the nanomechanical (elastic modulus) and morphological properties (collagen fibril diameter and D-Period) of the IMA were measured. Principal component analysis (PCA) was used to determine the relationship of nanomechanical and structural data with proteomics data (small leucine rich proteoglycans, SLRPs) [1] and patient metadata.

Results: PCA analysis shows that the nano-scale elastic modulus was one of the key variables which separated low and high PWV groups and was correlated with PWV. Furthermore, nano-scale alterations in adventitial collagen fibrils were evident. D-Period and collagen fibril diameter were found to be negatively correlated. Most SLRPs were closely grouped in the PCA analysis.

Conclusions: Although the IMA is not involved in the carotid-femoral pathway, patients with high PWV exhibited distinct alterations in the IMA at the nano-scale relative to those with low PWV. Our approach provides new insight into systemic structure-property changes in the vasculature, and also provides a novel method for characterizing small biopsy samples for arterial stiffening studies.

Open Access
This is an open access article distributed under the CC BY-NC license.

Reference

[1]ML Hansen, HC Beck, A Irmukhamedov, PS Jensen, MH Olsen, and LM Rasmussen, Proteome analysis of human arterial tissue discloses associations between the vascular content of small leucine-rich repeat proteoglycans and pulse wave velocity, Arterioscl. Thrombosis Vasc. Biol., Vol. 35, 2015, pp. 1896-1903.
Journal
Artery Research
Volume-Issue
24 - C
Pages
75 - 75
Publication Date
2018/12/04
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2018.10.040How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Riaz Akhtar
AU  - Zhuo Chang
AU  - Maria Lyck Hansen
AU  - Hans Christian Beck
AU  - Lars Melholt Rasmussen
PY  - 2018
DA  - 2018/12/04
TI  - 4.1 PROBING ARTERIAL STIFFNESS AT THE NANO-SCALE USING THE INTERNAL MAMMARY ARTERY AS A NOVEL TARGET
JO  - Artery Research
SP  - 75
EP  - 75
VL  - 24
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2018.10.040
DO  - 10.1016/j.artres.2018.10.040
ID  - Akhtar2018
ER  -