Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)

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Temperature Optimization and Inhibition Test of Lactobacillus acidophilus Bacteriocin Against Salmonella typhi Bacteria

Authors
Siska Telly Pratiwi, Muhammad Bagas Aditya, Endah Hamidah Abbas
Corresponding Author
Siska Telly Pratiwi
Available Online 24 July 2021.
DOI
10.2991/ahsr.k.210723.002How to use a DOI?
Keywords
bacteriocin, antibacterial, Lactobacillus acidophilus, Salmonella typhi, inhibition
Abstract

Typhoid fever is an infection of the digestive tract caused by Salmonella typhi bacteria. One of the Lactic Acid Bacteria (LAB) in the digestive tract is Lactobacillus acidophilus which has bacteriocins to protect the digestive tract. Bacteriocin is an antimicrobial protein compound that is bactericidal or bacteriostatic. Bacteriocin protein have an optimum temperature range to provide antimicrobial effect. The aimed of this study was determine the temperature optimization of L.acidophilus bacteriocins on S.typhi growth by agar diffusion method. In this research, the extraction of L.acidophilus ATCC 4356 bacteriocin were treated with a temperature of 40o, 60o, 80o, 100o, and 121oC. The results of research observations were carried out by measuring the diameter of the inhibition zone in each treatment. The result temperature optimization test for L.acidophilus bacteriocins did not give positive inhibition zone. This is due to several reasons, namely the storage and McFarland 0.5 standard setting is too small to inhibit S.typhi growth. This is evidenced by the preliminary test without McFarland arrangement which can provide 3mm diameter inhibition. Therefore, further research is needed related to the use of the Mcfarland standard and its bacteriocin storage.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
Series
Advances in Health Sciences Research
Publication Date
24 July 2021
ISBN
10.2991/ahsr.k.210723.002
ISSN
2468-5739
DOI
10.2991/ahsr.k.210723.002How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Siska Telly Pratiwi
AU  - Muhammad Bagas Aditya
AU  - Endah Hamidah Abbas
PY  - 2021
DA  - 2021/07/24
TI  - Temperature Optimization and Inhibition Test of Lactobacillus acidophilus Bacteriocin Against Salmonella typhi Bacteria
BT  - Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
PB  - Atlantis Press
SP  - 5
EP  - 9
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210723.002
DO  - 10.2991/ahsr.k.210723.002
ID  - Pratiwi2021
ER  -