Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)

Authors
Jiao Liu, Shaoping Fu, Yuqiang Guo, Shuo Wang, Ruijun Duan, Ruimei Li, Yuan Yao, Jianchun Guo
Corresponding Author
Jiao Liu
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.70How to use a DOI?
Keywords
cDNA Library, Yeast One-Hybrid, Cassava.
Abstract
Cassava is an important food and potential biofuel crop with starch-rich edible tuberous roots. It is tolerant to multiple abiotic stresses and can produce an acceptable yield under the adverse climatic and nutrient-poor soil. However, the molecular mechanisms of starch accumulation and the abiotic stress response involved in cassava are less known. In the present study, total RNA was isolated from the mixed leaves, stems, flowers, fruits and storage roots of cassava, and a good quality cDNA library was constructed. The titers of the resultant cDNA library were ~1.15×107 pfu/ml and an average insert size of ~1.5 kb. Therefore, the cDNA library constructed in this study was highly efficient. It can be used in the yeast one/two -hybrid systems to investigate both known and new interactions between DNA sequence of interest and transcription factors involved in the metabolic pathways and signaling transduction pathways of cassava.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.70How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Jiao Liu
AU  - Shaoping Fu
AU  - Yuqiang Guo
AU  - Shuo Wang
AU  - Ruijun Duan
AU  - Ruimei Li
AU  - Yuan Yao
AU  - Jianchun Guo
PY  - 2016/07
DA  - 2016/07
TI  - Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.70
DO  - https://doi.org/10.2991/bbe-16.2016.70
ID  - Liu2016/07
ER  -