Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)
Jiao Liu, Shaoping Fu, Yuqiang Guo, Shuo Wang, Ruijun Duan, Ruimei Li, Yuan Yao, Jianchun Guo
Available Online July 2016.
- https://doi.org/10.2991/bbe-16.2016.70How to use a DOI?
- cDNA Library, Yeast One-Hybrid, Cassava.
- Cassava is an important food and potential biofuel crop with starch-rich edible tuberous roots. It is tolerant to multiple abiotic stresses and can produce an acceptable yield under the adverse climatic and nutrient-poor soil. However, the molecular mechanisms of starch accumulation and the abiotic stress response involved in cassava are less known. In the present study, total RNA was isolated from the mixed leaves, stems, flowers, fruits and storage roots of cassava, and a good quality cDNA library was constructed. The titers of the resultant cDNA library were ~1.15×107 pfu/ml and an average insert size of ~1.5 kb. Therefore, the cDNA library constructed in this study was highly efficient. It can be used in the yeast one/two -hybrid systems to investigate both known and new interactions between DNA sequence of interest and transcription factors involved in the metabolic pathways and signaling transduction pathways of cassava.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Jiao Liu AU - Shaoping Fu AU - Yuqiang Guo AU - Shuo Wang AU - Ruijun Duan AU - Ruimei Li AU - Yuan Yao AU - Jianchun Guo PY - 2016/07 DA - 2016/07 TI - Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz) BT - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering PB - Atlantis Press SP - 452 EP - 455 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.70 DO - https://doi.org/10.2991/bbe-16.2016.70 ID - Liu2016/07 ER -