Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

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77 articles

Biotherapy Effect of Shenfuweikang Herbs on Gastric Carcinoma

Xiaoping Wang, Huanping Lin, Qiaoxia Wang, Bing Xu, Yi Li, Baoning Qi, Xuxu Wei, Pengfei Zhang
To study the biotherapy effect of Shenfuweikang herbs in treatment of gastric cancer, Kunming mice were grafted with a mouse gastric adenocarcinoma cell line MFC. Mice received different doses of Shefuweikang herbs after grafting. Tumor size was periodically measured and tumor weight was determined after...

Synthesis and Characterization of 3-butyryloxy-16-(β-naphthylmethylene)-5α-androstane-17-one

Hongqi Li, Qingwei Liu, Jueshu Fang, Yulong Wang
3-Butyryloxy-16-(β-naphthylmethylene)-5α-androstane-17-one, a novel androstanone derivative, was synthesized by aldol condensation between epiandrosterone and β-naphthaldehyde and subsequent esterification reaction. The structure of the new product was characterized by 1H NMR, 13C NMR, IR, UV and mass...

Temporal Expression MicroRNA-21 in Serum of Patients with Spinal Cord Injury

Hongyan Li, Dongxu Zhao, Minglei Zhang
Objective: To investigate the levels of microRNA-21 (miR-21) in the serum of patients with spinal cord injury (SCI) and to determine whether there was a correlation with degree of injury. Methods: Quantitative real-time reverse transcription–polymerase chain reaction( q-RT-PCR)was used to measure the...

Experimental Research on the Effect of Ginsenoside Rb1 in Curing Rats with Spinal Cord Ischemia-reperfusion Injury

Boyin Zhang, Dongxu Zhao, Chunyang Meng
Spinal cord ischemia-reperfusion injury (SCII), which was proposed by Allen in 1911, is the secondary damage of primary spinal cord injury and the main reason resulting in nerve injury complication and sequela [1]. Ginsenoside is one of the ingredients of Ginseng and the monomer composition being purified...

Anti-fatigue Effects of the Genistein Supplementation in Mice Subjected to Forced Swimming Test

Qiongli Hu
The present study was designed to evaluate the anti-fatigue effects of genistein in mice subjected to the forced swimming test. Mice were divided into four groups, i.e., one control group and three genistein treated groups. The treated groups were received doses of genistein (7, 15 and 30 mg/kg) dissolved...

Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to MMP-9,TIMP-1 of IgA Nephropathy Model Rat

Di Zou, Yinping Wang, Tianying Chang, Shoulin Zhang
Purpose:To study the method of tonifying kidney and spleen, detoxification of throat in treatment of IgA nephropathy. Methods:72 Wistar rats were randomly divided into blank group, model group, Lotensin group, tonifying the kidney and spleen, detoxification of method of high, medium and low dose group...

Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae

Kunli Zhao, Wei Guo, Dan Zhao, Yakun Zhang, Xiaoping Yan, Yujie Gao
A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of...

Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens

Yi Zhang, Lixue Wen, Haiyan Bao, Yingying Nie, Yan Feng, Zhilong Xiu
To activate cryptic secondary metabolic genes is a crucial issue in the search for new natural products from microorganisms. For this purpose, in the present study, a marine fungal strain Penicillium chrysogenum WP-1 was treated with dielectric barrier discharge plasma, chemical mutagens, and their combination....

Mitochondrial Toxicity of Tenofovir Dipivoxil Fumarate in HK-2 Cells

Qihui Luo, Junjun Zhao, Xinglei Feng, Zhengli Chen, Wen Zeng, Li Gong, Anchun Cheng, Yubo Shen, Chunmei Zhu
Nucleoside analogs have been shown to have mitochondrial toxicity. In vitro tests can evaluate the mechanisms and severity of the toxicity. Tenofovir dipivoxil fumarate (TDF) is a novel drug for type B hepatitis. In this study, HK-2 cells were used to evaluate the effect of TDF on mitochondrial toxicity...

Fasudil Protects Against the Myocardial Hypertrophy of Transverse Aortic Constriction Via Modulating the Expression of Caspase-3 and Bcl-2 Protein

Le Li, Hao Shang, Cailing Zhang, Houquan Tao, Jirkun Cheng
Objectives: To investigate the effects of fasudil (Fas) on myocardial hypertrophy in rats. Methods Rats were randomly divided into 5 groups: control , transverse aortic constriction (TAC) model, Fas (5mg/kg) plus TAC, TAC plus Fas(20mg/kg), and TAC plus captopril (Cap, positive drug). After administering...

Concentrations of Trace Elements in Different Types of Gallstones and Their Effects on Gallstone Formation

Yue Zhang, Chongyu Zhang, Da Guo, Xiaojun Huang, Junfa Xue, Jianming Ouyang
Purpose: To study the concentrations of trace metals and nonmetals elements in different types of gallstones (cholesterol-, pigment-, mixed- and calcium carbonate type) and to explore the formation mechanisms of gallstones. Method: Inductively coupled plasma emission spectrometer (ICP-AES) was used to...

The Correlation between the Treatment Time and Efficacy of Suspended Moxibustion on the Guanyuan Point for the Treatment of Primary Dysmenorrhea

Yongchun Hou, Zi Yan, Yaokun Xiong, Yanting Wan, Zhiyong Liu
Primary dysmenorrhea refers to lower abdominal pain, mild lumbago and discomfort in the lower abdomen during or before the menstrual period. Moxibustion has shown efficacy in the treatment of dysmenorrhea by removing blood stasis and relieving pain. The objective of the current study was to compare the...

Pereutaneous Nephrolithotomy in Elderly Patients with Renal Calculi

Fanping Meng, Jiantao Xian, Xu Zhou, Ji Gao
Objective: To explore the safety and efficacy of percutaneous nephrolithotomy (PCNL) for the treatment of renal calculi in elderly patients. Methods: Totally 57 elderly patients (mean age 69 years, ranged from 61 to 87 years) with renal calculi were treated by PCNL in our hospital from January 2013 to...

Treatment and Handling of Purulent Core Production in Facial Spatial Infection by VSD Technique

Lei Shi, Ruimin Liu, Qiaorong Liu
Objective: To discuss treatment of purulent core in facial spatial infection by VSD technique and handling effect; Methods: patients with drainage tube blockage by purulent core are selected as research objects among patients undergoing treatment of oral cavity facial spatial infection by VSD technique,...

Effects of Androgen on Female Individuals

Xiaoli Yan, Xintong Meng, Mingsan Miao
Objective: To investigate the effect of androgen on female individuals. Methods: In the physiological, pathological and pharmacological conditions, we summarize the role of androgen in female individuals, analyze its current research situation, and speculate the possible research direction. Results:...

Effects of Tonifying Kidney and Spleen, Detoxification of Throat for Th1 /Th2 of IgA Nephropathy Model Rats

Di Zou, Dongmei Zhang, Yinping Wang, Shoulin Zhang
Purposes:to investigate the effects of tonifying kidney and spleen, detoxification of throat for Th1 /Th2 of IgA nephropathy model rats.Methods : 72 rats were randomly divided into blank group, model group, Tripterygium wilfordii multi glycoside tablet group (control group), tonifying kidney and spleen,...

Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase

Ren Peng, Binwen Hong, Pan Zhou
A novel fungus which produced lipase extracellularly was isolated from soil. The strain was named after Candida tropicalis SD7 based on morphological features and ITS1-5.8S–ITS4 region sequences of ribosomal RNA. The fermentation conditions were optimized in order to improve lipase production of the...

Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to TNF-α, IL-6 of IgA Nephropathy Model Rat

Di Zou, Yinping Wang, Dongmei Zhang, Shoulin Zhang
Purpose: To study the effect and function mechanism of Tonifying Kidney and Spleen, Detoxification of Throat method on IgA nephropathy. Methods: the 72 Wistar rats were randomly divided into blank group, model group, Lotensin group, Tonifying kidney and spleen, detoxification of throat high, medium and...

The Osteoprotective Effect of Zhuanggu-Guanjie Pill in Orchidectomized Mice

Xiaomei Yuan, Yanan Bi, Zeman Yan, Hong Shi, Kun Zhou
To verify the osteoprotective effect of Zhuanggu-Guanjie (ZGGJ) in male mice. The male mice were randomly divided into 6 groups: control group (sham-operation), model group (orchidectomy group), three ZGGJ groups at doses of 0.77, 1.54 and 3.08g/kg, as well as Alendronate sodium group as positive control....

Vibration Analyses of a Single Osteoblast in Vitro using the Finite Element Method

Liping Wang, Hungyao Hsu, Cory J. Xian
In this study, the dynamic properties of a cell in vitro are analyzed using the finite element (FE) model. An idealized FE model of a bone cell is developed, and then FE analysis is performed to study its vibration in vitro. The first ten natural frequencies of the bone cell can be obtained using the...

Research on Embedded Electrical Impedance Measurement System

Xiaoyan Chen, Liang Sun, Meng Du
Based on S3C2440 processor and Linux3.6.6 kernel, wedeveloped an embedded impedance measurement system. This embedded system employ AD5933 as the impedance sensor, and data communication between AD5933 and S3C2440 processor is implemented with IIC communication protocol. In addition, the measurement...

A New Method to Identify Housekeeping Genes and Tissue Special Genes

Jixian Rao, Wei Liu, Hongwei Xie
Despite thedetection methods of HK genes and TS genes were various, without the standard database, we could not judge which one was the best classifier. In this paper, we built a standard database of HK genes and TS genes, reviewed and evaluated the existed methods. By analyzing the features of HK genes...

Electrical Kindling Rat Model Preparation and Analysis of EEG Signals

Lijuan Shi, Yi Yu, Yun Zhao, Liuyang Xu, Xinqi He, Juntang Lin
. Epilepsy (Epilepsy, EP) is a brain functional disorder caused by brain neurons abnormal discharge, however, the etiology and pathogenesis of epilepsy is still unclear. With the development of computer technology and digital signal processing technology, research and analysis of EEG signals is gradually...

Research on Functional Electrical Stimulation to Achieve Hand Gripping Action

Xinqi He, Lijuan Shi, Liuyang Xu, Yi Yu, Yun Zhao, Juntang Lin
. Functional Electrical Stimulation is a treatment method of using the output current of the functional electric stimulator to stimulate the nerves or muscles through the setting of functional electrical stimulator parameters. The motor function of the injured patients recovery the function of movement...

Acute Toxicity of Seleniferous Pleurotus Geesteranus Mycelia and Antioxidation against D-galactose-induced Mice

Junchen Chen, Li Wu, Hengsheng Shen, Minjie Weng, Pufu Lai
Liquid culture of seleniferous mycelia of Pleurotus geesteranus Singer (SeMyc-P) was evaluated for its acute toxicity in supplement application and antioxidant against D-galactose induced oxidation damaged mice. Maximum Tolerant Dose (MTD) of SeMyc-P was about 20g/kg bw for mice to define safety application...

Effects of Ozone Stress on Secondary Metabolism and Antioxidant Capacity in Soybean Roots

Tianhong Zhao, Wei Wang, Rongrong Tian, Yan Wang
In this paper, to examine the effects of elevated ozone on secondary metabolism and antioxidant capacity in soybean roots, as well as reactive oxygen species and antioxidant enzymes activity. Soybean grown in open top chambers (OTCs) were exposed to ambient air ([O3]=40nmol•mol-1) and elevated ozone...

Glyphosate Bioremediation of Contaminated Fish-pond Water by Paenibacillus sp. FUJX 401 from Industrial Activated Sludge

Guiming Fu, Ruyi Li, Xuehua Wu, Bin Gao, Xiaoqiang Yuan, Caixia Wan, Chengmei Liu
Six glyphosate (GP)-degrading strains were isolated from industrial activated sludge. Among them, strain FUJX 401 showed the highest GP degradability, and was identified as Paenibacillus sp. based on morphological observation, physiological biochemical characteristics, and 16S rRNA gene-sequence analysis....

Studies on Salt Tolerance of Bacillus Isolated from the Industrial Soy Sauce Residue

Zhenbo Xu, Xuerui Bao, Lili Ji, Jingjing Duan, Hepo Jin, Yang Deng, Lin Li, Bing Li
The annual output of soy sauce was reported to be more than 4.4 million tons in China, which would threaten the environment with its high salinity. The Bacillus in this study were isolated from soy sauce residue, which would contribute to its recycling. This study aims to investigate the external and...

Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche

Xue Han, Bing Han
Poplar as a woody-plant model is significant to improve the transformation efficiency of poplar. The effect of different leaf age as explants on transformation frequency was studied. The results exhibited that the higher transformation frequency was generated by 30-day-old leaf explants, compared with...

Properties of Oxidized Starch Prepared by Hydrogen Peroxide, Chlorine Dioxide and Sodium Hypochlorite

Bing Han
Chlorine peroxide, Sodium hypochlorite and hydrogen peroxide were chosen as oxidant to prepare potato oxidized starch, and the properties of oxidized starch were determined. The results showed that carbonyl content of oxidized starch prepared by H2O2 is 0.329%, much higher than other oxidized starch...

The Nursing Care of a Chinese Suicidal Patient with an Internal Jugular Vein Catheterization with Hemodialysis

Zhengping Qu, Di Zou, Lei Chen, Shoulin Zhang
Purposes: Summarize the experience of nursing care on a suicidal patient with an internal jugular vein catheterization with hemodialysis. Prevention of such adverse events, improving adherence of patient to hemodialysis. Method: According to the patient's condition, implement emergency care when in a...

A Novel, Non-Cytotoxic, Anti-Invasive Therapeutic Agent for Ovarian Cancer

S.D. Swenson, F.S. Markland, R. Minea
The 5-year survival is only about 30% when ovarian cancer (OC) is first detected at an advanced stage, which is the case for ~70% of women with OC. The combination of debulking surgery and cytotoxic chemotherapy is the primary treatment modality for OC. There has been much interest in the use of intraperitoneal...

Effects of Nano-selenium on Blood Biochemistry, Liver Antioxidant Activity and GPx-1 mRNA Expression in Rabbits

Shunyi Qin, Fu Chen, Fanghong Zhao, Tianming Jin, Jifei Ma
The experiment was conducted to compare the effect of nano-selenium and sodium selenite on blood biochemistry, liver antioxidant activity and GPx-1 mRNA expression in rabbit. Twenty-four 10-week-old New Zealand rabbits, female and male in half, were randomly divided into three groups. Rabbits in Group...

Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion

Yahong Huai, Shangzhong Xu
In order to study Trf 2 protein function in the sperm production, the Trf2 gene was studied and the protein function was predicted in this paper. Based on blast searches with the mRNA sequence of human Trf 2 and the bovine-EST databases, we used fragment assembly and RT-PCR technology to amplify sequence....

Influence of Daidzein and Daidzein Metabolites on Lifespan and Stress Resistance in Caenorhabditis Elegans

Ye Wang, Xiaolei Li, Conghui Zhang, Xiuling Wang
Caenorhabditis elegans (C. elegans) was used as an animal model to study the influence of isoflavone daidzein and daidzein metabolites, including dihydrodaidzein (DHD), O-desmethylangolensin (O-Dma) and equol, on lifespan in C. elegans at the concentration of 0.1 mmol/L. The results showed that equol...

Minocycline Attenuates Kidney Injury in a Rat Model Of Streptozotocin-Induced Diabetic Nephropathy

Hongping Yuan, Dongxu Zhao, Boyin Zhang
The effects of minocycline on the development of diabetic nephropathy (DN) in streptozotocin (STZ) induced diabetic rats were evaluated in this study. The diabetes rats with diabetic nephropathy (DN) were induced by STZ (55 mg/kg) injection. The experiment included 5 groups 1) normal, 2) normal plus...

Subcostal Transversus Abdominis Plane Block for Postoperative Analgesia

Kai Li, Ruizhu Liu, Lu Chen, Guoqing Zhao, Rangjuan Cao
To evaluate the effect of subcostal transversus abdominis plane (TAP) block on postoperative analgesia and short-term prognosis. Forty patients were divided into T and N groups. Ultrasound-guided bilateral subcostal TAP block was performed by injection of ropivacaine or normal saline using patient-controlled...

The Mechanism of Carthamin Yellow Injection Promote Acute Myocardial Infarction Infarct Border Zone Angiogenesis

Xiaomin Wang, Zhiyu Zhou, Yaokun Xiong, Jiangtao Wei, Yanting Wan, Fei Li, Zhiyong Liu
We observed the impact of carthamin yellow injection on revascularization in rats with acute myocardial infarction and discuss its mechanism. The left anterior descending branches of the coronary arteries of Wistar rats were ligatured, and the animal model with acute myocardial infarction was duplicated....

A New DNA Cryptography Algorithm Based on the Biological Puzzle and DNA Chip Techniques

Yunpeng Zhang, Zhenzhen Wang, Zhiwen Wang, Yasin Hasan Karanfil, Weidi Dai
Currently, a lot of theoretical and technical issues still need to be addressed regarding applications of DNA computing in the field of DNA cryptography. The current common DNA cryptography algorithms are basically based on traditional cryptography, integrate dintoexisting DNA technology, of which the...

An Improved Target-decoy Strategy for Evaluation of Database Search Engines and Quality Control Methods in Shotgun Proteomics

Xiaodong Feng, Jie Ma, Cheng Chang, Kunxian Shu, Yunping Zhu
With the advance of mass spectrometry and experimental techniques, proteome research has broken through the bottleneck of data generation, and a huge amount of mass spectrometry (MS) data has been accumulated rapidly in the past few years. Meanwhile, the lack of efficient data analysis and quality control...

Numerical Study on the Effect of Liquid Injection in Hepatic Microwave Ablation

Zhen Tian, Qun Nan, Xiaohui Nie, Tong Dong, Ruirui Wang
Purpose: The aim of this study is to research the impact of the liquid injection in hepatic microwave ablation (MWA) by using finite element method (FEM). Methods: We used the finite element method to simulate the MWA without liquid injection, with 0.9% NACL (saline) and with ethanol in turn. At the...

Diagnosing Small Cell Carcinoma of the Bladder

Hongyan Li, Hai Li, Yuanyuan Xing
Small cell carcinoma of the urethra (SCCB) is a rare yet highly aggressive malignancy. The pathogenesis of SCCB is poorly understood due to few large-scale cohort studies. Currently, SCCB is diagnosed primarily by morphological assessment. However, immunohistochemical staining can also play an important...

Ultrasonography-Guided Percutaneous Nephrolithotomy for the Treatment of Kidney Stone

Hongyan Li, Hai Li, Yuanyuan Xing
We examined the surgical outcomes of minimally invasive percutaneous nephrolithotomy (MPCNL) in complicating urolithiasis.10 patients were hospitalized for MPNCL due to upper tract urolithiasis. Calyx puncture was performed in the prone position under ultrasonographic guidance. The renal access route...

Application of Flexible Ureteroscope in Diagnosis and Treatment of Upper Urinary Tract Disease

Fanping Meng, Jiantao Xian, Xu Zhou, Ji Gao
Objective: To summarize the clinic experience of flexible ureteroscope in diagnosis and treatment of upper urinary tract disease. Method: From Nov 2013 to Dec 2015, 60 hematuresis cases underwent flexible ureteroscope for diagnosis and treatment of upper urinary tract. Clinical data was analyzed retrospectively....

PLS-DA Infrared Spectra Model of Citrus Leaves for the Characterization of Citrus Huanglongbing

Dongmei Chen, Xuejuan Lv, Huatang Wang, Jiali Liu, Xinnian Zeng
Citrus huanglongbing disease is an important disease of citrus species, which leads to the change of chemical composition in leaves. The infrared technique may be advantageous to characterize the chemical differences between Huanglongbing infected and healthy leaves rapidly and nondestructively. In this...

DNA-based Method for Identification of Species Origin of Honey and Edible Bird's Nest

Nyukling Chin, Siokpeng Kek, Meeichien Quek
Food traceability is increasingly important and identification of food source and its origin will give both consumers and industry players a better sense of security. The species origins of honey and edible bird's nest were identified using DNA-based method through procedures of DNA extraction, polymerase...

Feasibility Assessment of Low Iodine Flow Rate Imaging in Abdominal Contrast-enhanced CT

Ye Li, Huimao Zhang, Hongyan Li
To assess the feasibility of abdominal contrast-enhanced CT imaging using low iodine flow rate .40 consecutive patients referred for abdominal contrast-enhanced CT imaging of upper abdomen were enrolled in this study,and were randomized into 2 groups according to different injection protocols. 28 grams...

Research on Efficient Algorithm of Peptide Identification and Quantification in Proteomics

Zhen Wang, Jiyang Zhang, Yunping Zhu, Hongwei Xie
Because of its simple experiment design and low experiment cost, label-free quantitative technology based on mass spectrometry analysis is being used more and more widely. Aiming at peptide identification and quantification, which are pivotal step of the label-free quantitative analysis, we develop an...

The Application of Up-conversion Nanoparticles Materials in Cancer Diagnosis and Therapy

Yajuan Xu, Tao Luan, Baitao Zeng, Wenshu Liu
According to estimates by oncologists, if early tumor diagnosis and treatment in a timely manner can be achieved in the millimeter level or below, the cure rate of cancer will be able to increase to 80%-90%. With the rapid development and maturity of nano technology and scientific theory, Up-conversion...

Globulin Conjugated Fe3O4 Nanoparticles for Magnetic Hyperthermia

Viveka Kalidasan, Jun Ding
Magnetic hyperthermia is a non-invasive cancer treatment used synergistically with the existing cancer treatments. The challenges of an efficient magnetic hyperthermia are improved biocompatibility and enhanced heating characteristics. We have addressed both the challenges by fabricating globulin conjugated...