Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
Research on Embedded Electrical Impedance Measurement System
Xiaoyan Chen, Liang Sun, Meng Du
Based on S3C2440 processor and Linux3.6.6 kernel, wedeveloped an embedded impedance measurement system. This embedded system employ AD5933 as the impedance sensor, and data communication between AD5933 and S3C2440 processor is implemented with IIC communication protocol. In addition, the measurement...
Diagnosing Small Cell Carcinoma of the Bladder
Hongyan Li, Hai Li, Yuanyuan Xing
Small cell carcinoma of the urethra (SCCB) is a rare yet highly aggressive malignancy. The pathogenesis of SCCB is poorly understood due to few large-scale cohort studies. Currently, SCCB is diagnosed primarily by morphological assessment. However, immunohistochemical staining can also play an important...
Radiofrequency Signal in the Radiofrequency Ablation System
Ruirui Wang, Qun Nan, Zhen Tian, Xiaohui Nie, Tong Dong
. Radiofrequency ablation technique is one of the tumor hyperthermia methods and has been widely used in clinical treatment because of its small traumatic, better curative effect and others advantages. Recently, the common automatic control of radiofrequency ablation system is temperature control model...
Ultrasonography-Guided Percutaneous Nephrolithotomy for the Treatment of Kidney Stone
Hongyan Li, Hai Li, Yuanyuan Xing
We examined the surgical outcomes of minimally invasive percutaneous nephrolithotomy (MPCNL) in complicating urolithiasis.10 patients were hospitalized for MPNCL due to upper tract urolithiasis. Calyx puncture was performed in the prone position under ultrasonographic guidance. The renal access route...
A New Method to Identify Housekeeping Genes and Tissue Special Genes
Jixian Rao, Wei Liu, Hongwei Xie
Despite thedetection methods of HK genes and TS genes were various, without the standard database, we could not judge which one was the best classifier. In this paper, we built a standard database of HK genes and TS genes, reviewed and evaluated the existed methods. By analyzing the features of HK genes...
Exploration and Research on Relieving Feedback Inhibition for Aspartate Kinase form Corynebacterium Pekinense by using Site-Saturation Mutagenesis and High Throughput Screening
Li Fang, Longyang Wang, Chunlei Liu, Zhirui Zhang, Chen Chen, Weihong Min
This research investigated the aspartate kinase (AK) of Corynebacterium pekinense (CpAK). The amino acid residue A297, which is closely related to feedback inhibition, was selected for site-directed mutagenesis, high-throughput screening, and enzyme kinetic analysis. In this research, the enzyme activity...
On-demand Take of Dapoxetine for the Treatment of Lifelong Premature Ejaculation: Prospective, Randomized, Placebo Controlled Study
Hongyan Li, Yuanyuan Xing, Hai Li
The objective of this study is to assess the safety and efficacy of on-demand use of dapoxetine in treating lifelong premature ejaculation.This is a prospective, single center ,single blind, placebo controlled study. During the period of months between january 2015 and july 2015,60 patients diagnosed...
Application of Flexible Ureteroscope in Diagnosis and Treatment of Upper Urinary Tract Disease
Fanping Meng, Jiantao Xian, Xu Zhou, Ji Gao
Objective: To summarize the clinic experience of flexible ureteroscope in diagnosis and treatment of upper urinary tract disease. Method: From Nov 2013 to Dec 2015, 60 hematuresis cases underwent flexible ureteroscope for diagnosis and treatment of upper urinary tract. Clinical data was analyzed retrospectively....
Electrical Kindling Rat Model Preparation and Analysis of EEG Signals
Lijuan Shi, Yi Yu, Yun Zhao, Liuyang Xu, Xinqi He, Juntang Lin
. Epilepsy (Epilepsy, EP) is a brain functional disorder caused by brain neurons abnormal discharge, however, the etiology and pathogenesis of epilepsy is still unclear. With the development of computer technology and digital signal processing technology, research and analysis of EEG signals is gradually...
Research on Functional Electrical Stimulation to Achieve Hand Gripping Action
Xinqi He, Lijuan Shi, Liuyang Xu, Yi Yu, Yun Zhao, Juntang Lin
. Functional Electrical Stimulation is a treatment method of using the output current of the functional electric stimulator to stimulate the nerves or muscles through the setting of functional electrical stimulator parameters. The motor function of the injured patients recovery the function of movement...
SEMG Signals BP Neural Network Classification Based on Wavelet Packet Energy
Liuyang Xu, Lijuan Shi, Yi Yu, Xinqi He, Yun Zhao, Juntang Lin
The purpose of this paper is to study the different features of surface electromyography signals while subjects take different actions with their forearms. In this paper, the surface electromyography signals, recorded from some healthy volunteers under different actions (fist, fist exhibition, inside...
Sleep Spindle Detection Based on Complex Demodulation
Jiabin Li, Bei Wang, Yu Zhang
In this paper, we investigated the characteristic waveform of sleep spindle by using complex demodulation method (CDM). The ultimate purpose is to develop the automatic sleep spindle detection algorithm for overnight sleep data inspection. The main method includes four procedures. Firstly, the influences...
Synthesis and Detecting Cancer Studies of Up-Conversion Luminescent Materials
Tao Luan, Deming Han, Baitao Zeng, Lihui Zhao
In this article, designed and preparated rare earth luminescence nanoparticle through different core-shell and doping ratio, improving synthetic conditions, it has uniform size and good crystallinity. Modified nanoparticles with DTT , surface of the nanoparticles with sulphur and combine with oral cancer...
PLS-DA Infrared Spectra Model of Citrus Leaves for the Characterization of Citrus Huanglongbing
Dongmei Chen, Xuejuan Lv, Huatang Wang, Jiali Liu, Xinnian Zeng
Citrus huanglongbing disease is an important disease of citrus species, which leads to the change of chemical composition in leaves. The infrared technique may be advantageous to characterize the chemical differences between Huanglongbing infected and healthy leaves rapidly and nondestructively. In this...
Polydopamine-based Materials as Carriers for Drug Delivery
Xinyu Cui, Yuanyi Wang, Yuanyuan Yin, Miaojing Li
Polydopamine (PDA)–based material with tailored structures and properties are of particular interests due to their multifunctions and potential applications as new colloidal structures in diverse field. The past several years has witnessed a rapid increase of research concerning the new fabrication strategies,...
Biotherapy Effect of Shenfuweikang Herbs on Gastric Carcinoma
Xiaoping Wang, Huanping Lin, Qiaoxia Wang, Bing Xu, Yi Li, Baoning Qi, Xuxu Wei, Pengfei Zhang
To study the biotherapy effect of Shenfuweikang herbs in treatment of gastric cancer, Kunming mice were grafted with a mouse gastric adenocarcinoma cell line MFC. Mice received different doses of Shefuweikang herbs after grafting. Tumor size was periodically measured and tumor weight was determined after...
Synthesis and Characterization of 3-butyryloxy-16-(β-naphthylmethylene)-5α-androstane-17-one
Hongqi Li, Qingwei Liu, Jueshu Fang, Yulong Wang
3-Butyryloxy-16-(β-naphthylmethylene)-5α-androstane-17-one, a novel androstanone derivative, was synthesized by aldol condensation between epiandrosterone and β-naphthaldehyde and subsequent esterification reaction. The structure of the new product was characterized by 1H NMR, 13C NMR, IR, UV and mass...
Acute Toxicity of Seleniferous Pleurotus Geesteranus Mycelia and Antioxidation against D-galactose-induced Mice
Junchen Chen, Li Wu, Hengsheng Shen, Minjie Weng, Pufu Lai
Liquid culture of seleniferous mycelia of Pleurotus geesteranus Singer (SeMyc-P) was evaluated for its acute toxicity in supplement application and antioxidant against D-galactose induced oxidation damaged mice. Maximum Tolerant Dose (MTD) of SeMyc-P was about 20g/kg bw for mice to define safety application...
One-pot Synthesis of Diinosine Triphosphate and Tetraphosphate
Huashan Huang, Shanshan Gong
An efficient one-pot protocol for the synthesis of symmetrical diinosine triphosphate (Ip3I) and diinosine tetraphosphate (Ip4I) from inosine 5 -phosphoropiperidate has been developed. The experimental results indicated that the employment of DCI activation could notably promote the reaction rate and...
Temporal Expression MicroRNA-21 in Serum of Patients with Spinal Cord Injury
Hongyan Li, Dongxu Zhao, Minglei Zhang
Objective: To investigate the levels of microRNA-21 (miR-21) in the serum of patients with spinal cord injury (SCI) and to determine whether there was a correlation with degree of injury. Methods: Quantitative real-time reverse transcription–polymerase chain reaction( q-RT-PCR)was used to measure the...
Experimental Research on the Effect of Ginsenoside Rb1 in Curing Rats with Spinal Cord Ischemia-reperfusion Injury
Boyin Zhang, Dongxu Zhao, Chunyang Meng
Spinal cord ischemia-reperfusion injury (SCII), which was proposed by Allen in 1911, is the secondary damage of primary spinal cord injury and the main reason resulting in nerve injury complication and sequela . Ginsenoside is one of the ingredients of Ginseng and the monomer composition being purified...
Research on Efficient Algorithm of Peptide Identification and Quantification in Proteomics
Zhen Wang, Jiyang Zhang, Yunping Zhu, Hongwei Xie
Because of its simple experiment design and low experiment cost, label-free quantitative technology based on mass spectrometry analysis is being used more and more widely. Aiming at peptide identification and quantification, which are pivotal step of the label-free quantitative analysis, we develop an...
Anti-fatigue Effects of the Genistein Supplementation in Mice Subjected to Forced Swimming Test
The present study was designed to evaluate the anti-fatigue effects of genistein in mice subjected to the forced swimming test. Mice were divided into four groups, i.e., one control group and three genistein treated groups. The treated groups were received doses of genistein (7, 15 and 30 mg/kg) dissolved...
The Application of Up-conversion Nanoparticles Materials in Cancer Diagnosis and Therapy
Yajuan Xu, Tao Luan, Baitao Zeng, Wenshu Liu
According to estimates by oncologists, if early tumor diagnosis and treatment in a timely manner can be achieved in the millimeter level or below, the cure rate of cancer will be able to increase to 80%-90%. With the rapid development and maturity of nano technology and scientific theory, Up-conversion...
Effects of Androgen on Female Individuals
Xiaoli Yan, Xintong Meng, Mingsan Miao
Objective: To investigate the effect of androgen on female individuals. Methods: In the physiological, pathological and pharmacological conditions, we summarize the role of androgen in female individuals, analyze its current research situation, and speculate the possible research direction. Results:...
Molecular Mechanism of Traditional Chinese Medicine Treatment of BPH based on bFGF/ERK and TGF-β /Smad Pathway
Xiaoli Yan, Lin Guo, Mingsan Miao
Objective: To investigate the molecular mechanism of benign prostatic hyperplasia (BPH) and the characteristics of traditional Chinese medicine in the treatment of BPH. Method: Around bFGF/ERK, TGF-β/Smad pathway and related apoptosis gene expression, By analyzing the expression of TGF-β, bFGF and their...
A Novel, Non-Cytotoxic, Anti-Invasive Therapeutic Agent for Ovarian Cancer
S.D. Swenson, F.S. Markland, R. Minea
The 5-year survival is only about 30% when ovarian cancer (OC) is first detected at an advanced stage, which is the case for ~70% of women with OC. The combination of debulking surgery and cytotoxic chemotherapy is the primary treatment modality for OC. There has been much interest in the use of intraperitoneal...
Effects of Nano-selenium on Blood Biochemistry, Liver Antioxidant Activity and GPx-1 mRNA Expression in Rabbits
Shunyi Qin, Fu Chen, Fanghong Zhao, Tianming Jin, Jifei Ma
The experiment was conducted to compare the effect of nano-selenium and sodium selenite on blood biochemistry, liver antioxidant activity and GPx-1 mRNA expression in rabbit. Twenty-four 10-week-old New Zealand rabbits, female and male in half, were randomly divided into three groups. Rabbits in Group...
Globulin Conjugated Fe3O4 Nanoparticles for Magnetic Hyperthermia
Viveka Kalidasan, Jun Ding
Magnetic hyperthermia is a non-invasive cancer treatment used synergistically with the existing cancer treatments. The challenges of an efficient magnetic hyperthermia are improved biocompatibility and enhanced heating characteristics. We have addressed both the challenges by fabricating globulin conjugated...
Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion
Yahong Huai, Shangzhong Xu
In order to study Trf 2 protein function in the sperm production, the Trf2 gene was studied and the protein function was predicted in this paper. Based on blast searches with the mRNA sequence of human Trf 2 and the bovine-EST databases, we used fragment assembly and RT-PCR technology to amplify sequence....
Kinetics of Tyrosinase Catalyzing Reaction Activated by 3-O-Caffeoylquinic Acid
Shengzhao Gong, Qingsheng Chen, Shimei Li
The effect of 3-O-caffeoylquinic acid on the monophenolase and diphenolase activity was tested. The experimental results showed that the activation effected of 3-O-caffeoylquinic acid on both monophenolase and diphenolase activity .3-O-caffeoylquinic acid concentration leading 50% activity increase (AC50)...
Effects of Tonifying Kidney and Spleen, Detoxification of Throat for Th1 /Th2 of IgA Nephropathy Model Rats
Di Zou, Dongmei Zhang, Yinping Wang, Shoulin Zhang
Purposes:to investigate the effects of tonifying kidney and spleen, detoxification of throat for Th1 /Th2 of IgA nephropathy model rats.Methods : 72 rats were randomly divided into blank group, model group, Tripterygium wilfordii multi glycoside tablet group (control group), tonifying kidney and spleen,...
Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase
Ren Peng, Binwen Hong, Pan Zhou
A novel fungus which produced lipase extracellularly was isolated from soil. The strain was named after Candida tropicalis SD7 based on morphological features and ITS1-5.8S–ITS4 region sequences of ribosomal RNA. The fermentation conditions were optimized in order to improve lipase production of the...
Influence of 660 and 830 nm Laser Irradiation on Genetic Profile of Extracellular Matrix Proteins in Diabetic Wounded Human Skin Fibroblast Cells
The extracellular matrix (ECM) provides tissue structural integrity and its synthesis plays a crucial role in wound healing. Impaired wound healing ensues following the destruction of the ECM, or the inhibition of its synthesis. An imbalance in ECM synthesis and degradation is seen in patients with diabetes....
Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to MMP-9,TIMP-1 of IgA Nephropathy Model Rat
Di Zou, Yinping Wang, Tianying Chang, Shoulin Zhang
Purpose:To study the method of tonifying kidney and spleen, detoxification of throat in treatment of IgA nephropathy. Methods:72 Wistar rats were randomly divided into blank group, model group, Lotensin group, tonifying the kidney and spleen, detoxification of method of high, medium and low dose group...
The Preparation and Research of Small Molecular Group Water
Pei Jiang, Qingshan Li, Mingyang Lian
The qualities of water are closely related with human health. Small molecular group water which contains 5-6 water molecules plays an important role to human body health. This paper reviewed the pure water was treated by the micro-nano powder of the Guhai rock to prepare a kind of small molecular group...
Influence of Tonifying Kidney and Spleen, Detoxification of Throat Method to TNF-α, IL-6 of IgA Nephropathy Model Rat
Di Zou, Yinping Wang, Dongmei Zhang, Shoulin Zhang
Purpose: To study the effect and function mechanism of Tonifying Kidney and Spleen, Detoxification of Throat method on IgA nephropathy. Methods: the 72 Wistar rats were randomly divided into blank group, model group, Lotensin group, Tonifying kidney and spleen, detoxification of throat high, medium and...
Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae
Kunli Zhao, Wei Guo, Dan Zhao, Yakun Zhang, Xiaoping Yan, Yujie Gao
A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of...
Influence of Daidzein and Daidzein Metabolites on Lifespan and Stress Resistance in Caenorhabditis Elegans
Ye Wang, Xiaolei Li, Conghui Zhang, Xiuling Wang
Caenorhabditis elegans (C. elegans) was used as an animal model to study the influence of isoflavone daidzein and daidzein metabolites, including dihydrodaidzein (DHD), O-desmethylangolensin (O-Dma) and equol, on lifespan in C. elegans at the concentration of 0.1 mmol/L. The results showed that equol...
Secondary Metabolism Variation of a Marine Fungus Following Treatment with Dielectric Barrier Discharge Plasma and Chemical Mutagens
Yi Zhang, Lixue Wen, Haiyan Bao, Yingying Nie, Yan Feng, Zhilong Xiu
To activate cryptic secondary metabolic genes is a crucial issue in the search for new natural products from microorganisms. For this purpose, in the present study, a marine fungal strain Penicillium chrysogenum WP-1 was treated with dielectric barrier discharge plasma, chemical mutagens, and their combination....
Minocycline Attenuates Kidney Injury in a Rat Model Of Streptozotocin-Induced Diabetic Nephropathy
Hongping Yuan, Dongxu Zhao, Boyin Zhang
The effects of minocycline on the development of diabetic nephropathy (DN) in streptozotocin (STZ) induced diabetic rats were evaluated in this study. The diabetes rats with diabetic nephropathy (DN) were induced by STZ (55 mg/kg) injection. The experiment included 5 groups 1) normal, 2) normal plus...
Subcostal Transversus Abdominis Plane Block for Postoperative Analgesia
Kai Li, Ruizhu Liu, Lu Chen, Guoqing Zhao, Rangjuan Cao
To evaluate the effect of subcostal transversus abdominis plane (TAP) block on postoperative analgesia and short-term prognosis. Forty patients were divided into T and N groups. Ultrasound-guided bilateral subcostal TAP block was performed by injection of ropivacaine or normal saline using patient-controlled...
Mitochondrial Toxicity of Tenofovir Dipivoxil Fumarate in HK-2 Cells
Qihui Luo, Junjun Zhao, Xinglei Feng, Zhengli Chen, Wen Zeng, Li Gong, Anchun Cheng, Yubo Shen, Chunmei Zhu
Nucleoside analogs have been shown to have mitochondrial toxicity. In vitro tests can evaluate the mechanisms and severity of the toxicity. Tenofovir dipivoxil fumarate (TDF) is a novel drug for type B hepatitis. In this study, HK-2 cells were used to evaluate the effect of TDF on mitochondrial toxicity...
The Osteoprotective Effect of Zhuanggu-Guanjie Pill in Orchidectomized Mice
Xiaomei Yuan, Yanan Bi, Zeman Yan, Hong Shi, Kun Zhou
To verify the osteoprotective effect of Zhuanggu-Guanjie (ZGGJ) in male mice. The male mice were randomly divided into 6 groups: control group (sham-operation), model group (orchidectomy group), three ZGGJ groups at doses of 0.77, 1.54 and 3.08g/kg, as well as Alendronate sodium group as positive control....
Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells
Poonam Bhadja, Kai Yu, Xiuqun Yao, Jianming Ouyang
Objective: This study aims to compare the cytotoxicity and adhesion of submicron calcium oxalate monohydrate (COM) and dehydrate (COD) crystals toward African green monkey renal epithelial cells (Vero) to reveal the mechanism of kidney stone formation at cellular level. Methods: The injury effect of...
Composition Difference of Cholesterol-, Pigment- and Mixed-type Gallstones, and Their Formation Mechanisms
Yue Zhang, Qin Kui, Cenyin Rao, Xiaojun Huang, Junfa Xue, Jianming Ouyang
Objective: The chemical compositions and formation mechanisms of different types of gallstones (cholesterol-, pigment-, and mixed-type) were studied. Methods: The components of gallstones were identified by Fourier transform infrared spectroscopy (FT-IR). The contents of cholesterol, bilirubin and bile...
Fasudil Protects Against the Myocardial Hypertrophy of Transverse Aortic Constriction Via Modulating the Expression of Caspase-3 and Bcl-2 Protein
Le Li, Hao Shang, Cailing Zhang, Houquan Tao, Jirkun Cheng
Objectives: To investigate the effects of fasudil (Fas) on myocardial hypertrophy in rats. Methods Rats were randomly divided into 5 groups: control , transverse aortic constriction (TAC) model, Fas (5mg/kg) plus TAC, TAC plus Fas(20mg/kg), and TAC plus captopril (Cap, positive drug). After administering...
Clinical Observation of 29 Cases of Tonifying Kidney and Spleen, Detoxification of Throat Traditional Chinese Medicine in the Treatment of IgA Nephropathy
Di Zou, Hongan Wang, Yinping Wang, Shoulin Zhang
Purpose:To observe the clinical effect of tonifying kidney and spleen, detoxification of throat traditional Chinese medicine in the treatment of IgA nephropathy. Methods:60 patients with IgA nephropathy were randomly divided into treatment group and control group with 30 cases in each.The treatment group...
Clinical Investigation on Gouqi Fushen Pills for Chronic Nephritis
Di Zou, Chengji Cui, Yinping Wang, Shoulin Zhang
Purpose:Observe the effectiveness and security of Gouqi fushen pills treating chronic nephritis. Method:Randomly divide 230 patients with chronic glomerular nephritis into two groups, treatment group and comparison group, each of 115 cases. Both groups orally take benazepril hydrochloride 10mg once a...
A Muscle-Specific Rehabilitation Training Method of the Human Shoulder Based on the Optimal Load Orientation Concept
Chao Nie, Zhibin Song, Rongjie Kang, Jian S. Dai
In order to implement the high-efficiency resistance training for a specific muscle of human shoulders using the rehabilitation robots, a muscle-specific rehabilitation training method based on the optimal load orientation concept (OLOC) was proposed. A 3D mathematical musculoskeletal model of the shoulder...
Concentrations of Trace Elements in Different Types of Gallstones and Their Effects on Gallstone Formation
Yue Zhang, Chongyu Zhang, Da Guo, Xiaojun Huang, Junfa Xue, Jianming Ouyang
Purpose: To study the concentrations of trace metals and nonmetals elements in different types of gallstones (cholesterol-, pigment-, mixed- and calcium carbonate type) and to explore the formation mechanisms of gallstones. Method: Inductively coupled plasma emission spectrometer (ICP-AES) was used to...
The Mechanism of Carthamin Yellow Injection Promote Acute Myocardial Infarction Infarct Border Zone Angiogenesis
Xiaomin Wang, Zhiyu Zhou, Yaokun Xiong, Jiangtao Wei, Yanting Wan, Fei Li, Zhiyong Liu
We observed the impact of carthamin yellow injection on revascularization in rats with acute myocardial infarction and discuss its mechanism. The left anterior descending branches of the coronary arteries of Wistar rats were ligatured, and the animal model with acute myocardial infarction was duplicated....
The Correlation between the Treatment Time and Efficacy of Suspended Moxibustion on the Guanyuan Point for the Treatment of Primary Dysmenorrhea
Yongchun Hou, Zi Yan, Yaokun Xiong, Yanting Wan, Zhiyong Liu
Primary dysmenorrhea refers to lower abdominal pain, mild lumbago and discomfort in the lower abdomen during or before the menstrual period. Moxibustion has shown efficacy in the treatment of dysmenorrhea by removing blood stasis and relieving pain. The objective of the current study was to compare the...
Research Progress on the Relationship between Interleukins Gene Polymorphism and Type 2 Diabetes
Yang Li, Miaojing Li, Xinyu Cui, Shengzhong Rong, Ge Jin, Hui Zhang, Yingying Chen, Qing Ye, Yuda Wang, Yue Guan
Inflammation plays a significant role in the etiology and pathogenesis of type 2 diabetes mellitus (T2DM).Many single nucleotide polymorphisms (SNPs) in various genes including inflammatory cytokines have been reported as a risk for T2DM.Interleukins (IL) such as IL - 6, IL - 10, IL - 18 and IL – 1 are...
A New DNA Cryptography Algorithm Based on the Biological Puzzle and DNA Chip Techniques
Yunpeng Zhang, Zhenzhen Wang, Zhiwen Wang, Yasin Hasan Karanfil, Weidi Dai
Currently, a lot of theoretical and technical issues still need to be addressed regarding applications of DNA computing in the field of DNA cryptography. The current common DNA cryptography algorithms are basically based on traditional cryptography, integrate dintoexisting DNA technology, of which the...
An Improved Target-decoy Strategy for Evaluation of Database Search Engines and Quality Control Methods in Shotgun Proteomics
Xiaodong Feng, Jie Ma, Cheng Chang, Kunxian Shu, Yunping Zhu
With the advance of mass spectrometry and experimental techniques, proteome research has broken through the bottleneck of data generation, and a huge amount of mass spectrometry (MS) data has been accumulated rapidly in the past few years. Meanwhile, the lack of efficient data analysis and quality control...
Numerical Study on the Effect of Liquid Injection in Hepatic Microwave Ablation
Zhen Tian, Qun Nan, Xiaohui Nie, Tong Dong, Ruirui Wang
Purpose: The aim of this study is to research the impact of the liquid injection in hepatic microwave ablation (MWA) by using finite element method (FEM). Methods: We used the finite element method to simulate the MWA without liquid injection, with 0.9% NACL (saline) and with ethanol in turn. At the...
Vibration Analyses of a Single Osteoblast in Vitro using the Finite Element Method
Liping Wang, Hungyao Hsu, Cory J. Xian
In this study, the dynamic properties of a cell in vitro are analyzed using the finite element (FE) model. An idealized FE model of a bone cell is developed, and then FE analysis is performed to study its vibration in vitro. The first ten natural frequencies of the bone cell can be obtained using the...
Network Analysis Reveals the Connection Between Chronic Pancreatitis and Pancreatic Cancer
Lisha Li, Heping Wang, Yulin Li
The relationship between chronic pancreatitis and pancreatic cancer needs further exploration in a system view. We constructed chronic pancreatitis and pancreatic cancer network based on gene expression data and protein-protein interaction, and then analyzed the global topological features of chronic...
Experimental Study of Bone Marrow Stem Cell Inducing Osteogenesis on SLA Titanium Mesh Surfac
Lei Shi, Lan Yang, Ruimin Liu, Feng Tao, Qiaorong Liu
Objective: Through animal experiments to understand medical titanium mesh in the sandblasting acid corrosion (SLA) before and after processing and surface coverage of BMSCs cell membrane after the effect of inducing osteogenesis, for medical titanium net covering the bone marrow stem cells after surface...
Age-related Senescence of Rat Bone Marrow-derived Mesenchymal Stem Cells
Xu He, Cao Ma, Chenchen Pi, Yue Yang, Lin Lin, Yingai Shi, Yulin Li
To study whether bone marrow-derived mesenchymal stem cells (MSCs) from aged rat present cell senescence. In the present study, cell morphology were observed, and cell proliferation and cell cycle were analyzed. The natural senescence of MSCs from aged rat was testified by investigation of SA- -gal activity,...
Enhanced Milk-Clotting Enzyme Production of Mucor Miehei Mutated by UV and LiCl
Weibing Zhang, Zhongming Zhang, Tuo Yao
Milk clotting enzyme is an important enzyme in cheese industry, to obtain an industrial strain with high milk-clotting enzyme activity, Mucor miehei was used as the origin strain and mutagenized by Ultraviolet (UV) and UV combining lithium chloride(LiCl), respectively. The result shows that lower concentrations...
Selection of Potential Probiotic Strains Isolated from Human Intestinal Tract and Traditional Ferment Milk
Chaohui Xue, Chun Yue, Xueguo Liu, Le Yuan
Diarrhea is the main reason of human deaths in developing countries, which also cause world-wide infant mortality. Diarrhoea occurs world-wide and causes 4% of all deaths and 5% of health loss to disability. It is most commonly caused by gastrointestinal infections which kill around 1.5 million people...
DNA-based Method for Identification of Species Origin of Honey and Edible Bird's Nest
Nyukling Chin, Siokpeng Kek, Meeichien Quek
Food traceability is increasingly important and identification of food source and its origin will give both consumers and industry players a better sense of security. The species origins of honey and edible bird's nest were identified using DNA-based method through procedures of DNA extraction, polymerase...
Effects of Ozone Stress on Secondary Metabolism and Antioxidant Capacity in Soybean Roots
Tianhong Zhao, Wei Wang, Rongrong Tian, Yan Wang
In this paper, to examine the effects of elevated ozone on secondary metabolism and antioxidant capacity in soybean roots, as well as reactive oxygen species and antioxidant enzymes activity. Soybean grown in open top chambers (OTCs) were exposed to ambient air ([O3]=40nmol•mol-1) and elevated ozone...
Glyphosate Bioremediation of Contaminated Fish-pond Water by Paenibacillus sp. FUJX 401 from Industrial Activated Sludge
Guiming Fu, Ruyi Li, Xuehua Wu, Bin Gao, Xiaoqiang Yuan, Caixia Wan, Chengmei Liu
Six glyphosate (GP)-degrading strains were isolated from industrial activated sludge. Among them, strain FUJX 401 showed the highest GP degradability, and was identified as Paenibacillus sp. based on morphological observation, physiological biochemical characteristics, and 16S rRNA gene-sequence analysis....
Studies on Salt Tolerance of Bacillus Isolated from the Industrial Soy Sauce Residue
Zhenbo Xu, Xuerui Bao, Lili Ji, Jingjing Duan, Hepo Jin, Yang Deng, Lin Li, Bing Li
The annual output of soy sauce was reported to be more than 4.4 million tons in China, which would threaten the environment with its high salinity. The Bacillus in this study were isolated from soy sauce residue, which would contribute to its recycling. This study aims to investigate the external and...
Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche
Xue Han, Bing Han
Poplar as a woody-plant model is significant to improve the transformation efficiency of poplar. The effect of different leaf age as explants on transformation frequency was studied. The results exhibited that the higher transformation frequency was generated by 30-day-old leaf explants, compared with...
Properties of Oxidized Starch Prepared by Hydrogen Peroxide, Chlorine Dioxide and Sodium Hypochlorite
Chlorine peroxide, Sodium hypochlorite and hydrogen peroxide were chosen as oxidant to prepare potato oxidized starch, and the properties of oxidized starch were determined. The results showed that carbonyl content of oxidized starch prepared by H2O2 is 0.329%, much higher than other oxidized starch...
Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)
Jiao Liu, Shaoping Fu, Yuqiang Guo, Shuo Wang, Ruijun Duan, Ruimei Li, Yuan Yao, Jianchun Guo
Cassava is an important food and potential biofuel crop with starch-rich edible tuberous roots. It is tolerant to multiple abiotic stresses and can produce an acceptable yield under the adverse climatic and nutrient-poor soil. However, the molecular mechanisms of starch accumulation and the abiotic stress...
Pereutaneous Nephrolithotomy in Elderly Patients with Renal Calculi
Fanping Meng, Jiantao Xian, Xu Zhou, Ji Gao
Objective: To explore the safety and efficacy of percutaneous nephrolithotomy (PCNL) for the treatment of renal calculi in elderly patients. Methods: Totally 57 elderly patients (mean age 69 years, ranged from 61 to 87 years) with renal calculi were treated by PCNL in our hospital from January 2013 to...
The Nursing Care of a Chinese Suicidal Patient with an Internal Jugular Vein Catheterization with Hemodialysis
Zhengping Qu, Di Zou, Lei Chen, Shoulin Zhang
Purposes: Summarize the experience of nursing care on a suicidal patient with an internal jugular vein catheterization with hemodialysis. Prevention of such adverse events, improving adherence of patient to hemodialysis. Method: According to the patient's condition, implement emergency care when in a...
Study on the Brain Stereotaxic Method of Carp Aquatic Animal-robot
Yong Peng, Congshan Guo, Yangyang Su, Zhanqiu Wang, Pei Hua Su, Dan Du, Weichao Shen, Yakun Ju, Ru Tian, Xiangqian Zhou, Fan Zhan, Jie Li, Lijuan Ying
[Purposes] Our aim is to explore the brain stereotaxic method of aquatic animal-robots. [Methods] Carps were anesthetized by Eugenol. The carp skulls and brain were observed by the method of anatomy. Carp brain structure and size were obtained by three-dimension images which were structured by CT. The...
Treatment and Handling of Purulent Core Production in Facial Spatial Infection by VSD Technique
Lei Shi, Ruimin Liu, Qiaorong Liu
Objective: To discuss treatment of purulent core in facial spatial infection by VSD technique and handling effect; Methods: patients with drainage tube blockage by purulent core are selected as research objects among patients undergoing treatment of oral cavity facial spatial infection by VSD technique,...
Feasibility Assessment of Low Iodine Flow Rate Imaging in Abdominal Contrast-enhanced CT
Ye Li, Huimao Zhang, Hongyan Li
To assess the feasibility of abdominal contrast-enhanced CT imaging using low iodine flow rate .40 consecutive patients referred for abdominal contrast-enhanced CT imaging of upper abdomen were enrolled in this study,and were randomized into 2 groups according to different injection protocols. 28 grams...
A Novel Method of HCC Pathological Images Recognition based on Morphological Distribution of Liver Cell Cords
Aoshuang Dong, Su Jiang, Huiyan Jiang, Keming Mao
The morbidity of hepatocellular carcinoma (HCC), which has become a significant killer to human life, is getting higher over the years. Not only the shape and structure of liver cells are abnormal in pathological images, but also liver cell cords show some unusual phenomena, such as incrassation and...
Target Control Infusion (TCI) Computer-Assisted Pump and Bispectral Index (BIS ) Monitoring Sedation System in Elderly Patients during Endoscopic Retrograde Cholangiopancreateography (ERCP)
Kai Li, Bin Zhang, Ying Zhang, Fang Wang, Chunmei Chu
Objectives: The aim of this study was to investigate the safety and efficacy of propofol sedation with a target-controlled infusion (TCI) computer-assisted pump and bispectral index (BIS) monitoring in elderly patients during endoscopic retrograde cholangiopancreateography (ERCP). Methods: Included were...