Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae
Kunli Zhao, Wei Guo, Dan Zhao, Yakun Zhang, Xiaoping Yan, Yujie Gao
Available Online July 2016.
- 10.2991/bbe-16.2016.38How to use a DOI?
- Bacillus thuringiensis, Cry gene,Infected Hyphantria cunea, Biological control.
A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of cry9Ea10 was cloned and sequenced. The engineering strain of Bt carrying cry9Ea10gene wasconstructed successfully. SDS-PAGE analysis confirmed that the expression of cry9Ea10 as ~130kDa protein. And Cry9Ea10 protein produced by Bt engineeringstrainexhibited a high toxicity to H.cunealarvae.
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Kunli Zhao AU - Wei Guo AU - Dan Zhao AU - Yakun Zhang AU - Xiaoping Yan AU - Yujie Gao PY - 2016/07 DA - 2016/07 TI - Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae BT - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering PB - Atlantis Press SP - 226 EP - 231 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.38 DO - 10.2991/bbe-16.2016.38 ID - Zhao2016/07 ER -