Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae

Authors
Kunli Zhao, Wei Guo, Dan Zhao, Yakun Zhang, Xiaoping Yan, Yujie Gao
Corresponding Author
Kunli Zhao
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.38How to use a DOI?
Keywords
Bacillus thuringiensis, Cry gene,Infected Hyphantria cunea, Biological control.
Abstract
A novelBacillus thuringiensis(Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes ofGZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of cry9Ea10 was cloned and sequenced. The engineering strain of Bt carrying cry9Ea10gene wasconstructed successfully. SDS-PAGE analysis confirmed that the expression of cry9Ea10 as ~130kDa protein. And Cry9Ea10 protein produced by Bt engineeringstrainexhibited a high toxicity to H.cunealarvae.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.38How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Kunli Zhao
AU  - Wei Guo
AU  - Dan Zhao
AU  - Yakun Zhang
AU  - Xiaoping Yan
AU  - Yujie Gao
PY  - 2016/07
DA  - 2016/07
TI  - Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.38
DO  - https://doi.org/10.2991/bbe-16.2016.38
ID  - Zhao2016/07
ER  -