Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells
Poonam Bhadja, Kai Yu, Xiuqun Yao, Jianming Ouyang
Available Online July 2016.
- https://doi.org/10.2991/bbe-16.2016.45How to use a DOI?
- Submicron Crystal, Concentration Effect, Cell Injury, Crystal Adhesion.
- Objective: This study aims to compare the cytotoxicity and adhesion of submicron calcium oxalate monohydrate (COM) and dehydrate (COD) crystals toward African green monkey renal epithelial cells (Vero) to reveal the mechanism of kidney stone formation at cellular level. Methods: The injury effect of COM and COD crystals in Vero was investigated by detecting the change of cell viability, lactate dehydrogenase (LDH) content and hyaluronan (HA) expression amount. The adhesion of crystals on cell surface was observed using scanning electron microscope; and the crystal adhesion amount was measured using inductively coupled plasma emission spectrometer. Results: The cellular injury induced by crystals was in a dose-dependent manner in the concentration range of 100~400 g/mL; COM caused more serious injury than COD, the adhesion amount of COM on Vero was significantly greater than COD. Conclusions: The damage of submicron COM is larger than submicron COD, the adhered COM crystals are more easily aggregated. The results in this paper indicated that the presence of high concentration of COM crystals in urine is more likely to increase the risk of stone formation than COD crystals.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Poonam Bhadja AU - Kai Yu AU - Xiuqun Yao AU - Jianming Ouyang PY - 2016/07 DA - 2016/07 TI - Injury Mechanism of Sub-micron Calcium Oxalate Monohydrate and Dihydrate Crystals on Renal Epithelial Cells PB - Atlantis Press SP - 283 EP - 289 SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.45 DO - https://doi.org/10.2991/bbe-16.2016.45 ID - Bhadja2016/07 ER -