Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase
- Ren Peng, Binwen Hong, Pan Zhou
- Corresponding Author
- Ren Peng
Available Online July 2016.
- https://doi.org/10.2991/bbe-16.2016.33How to use a DOI?
- Lipase, Screening, Organic solvents tolerance, Fermentation.
- A novel fungus which produced lipase extracellularly was isolated from soil. The strain was named after Candida tropicalis SD7 based on morphological features and ITS1-5.8S–ITS4 region sequences of ribosomal RNA. The fermentation conditions were optimized in order to improve lipase production of the strain. The maximum lipase activity was achieved at 72h. The optimum lipase production was observed in the medium with glycerol as carbon source and peptone as nitrogen source through submerged fermentation at 30°C and initial pH 7.0. The lipase showed stability in the presence of hydrophobic organic solvents such as methylbenzene, petroleum ether, isooctane and n-hexane. It was moderately stable in polar solvents, including DMSO, acetone, acetonitrile and methanol.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ren Peng AU - Binwen Hong AU - Pan Zhou PY - 2016/07 DA - 2016/07 TI - Screening and Production Optimization of a New Organic Solvent-Tolerance Fungal Lipase BT - International Conference on Biomedical and Biological Engineering PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.33 DO - https://doi.org/10.2991/bbe-16.2016.33 ID - Peng2016/07 ER -