Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Enhanced Milk-Clotting Enzyme Production of Mucor Miehei Mutated by UV and LiCl

Authors
Weibing Zhang, Zhongming Zhang, Tuo Yao
Corresponding Author
Weibing Zhang
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.62How to use a DOI?
Keywords
Milk-clotting enzyme, Mucor miehei, Mutation.
Abstract
Milk clotting enzyme is an important enzyme in cheese industry, to obtain an industrial strain with high milk-clotting enzyme activity, Mucor miehei was used as the origin strain and mutagenized by Ultraviolet (UV) and UV combining lithium chloride(LiCl), respectively. The result shows that lower concentrations of LiCl can increase the survival rate of the spores treated with UV, while higher concentration of LiCl can increase the mortality of the spores treated with UV. The highest positive rate (reached 32.17%) was achieved in the compound mutation, which was higher than that of the UV mutagenesis (21.45%). On the basis of multiple mutation, a mutant UV-LiCl-6 was screened. The milk-clotting activity of UV-LiCl-6 reached 2703.58 SU/mL and was 57.70% higher than that of the parent strain. Results of the pass-generation test indicated that the strain had good genetic stability and may be ideal strains for the production of milk-clotting enzyme. Additionally, it was demonstrated that compound mutagenesis with UV and LiCl is an effective mutation method for the breeding of enzyme-producing strains.
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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.62How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Weibing Zhang
AU  - Zhongming Zhang
AU  - Tuo Yao
PY  - 2016/07
DA  - 2016/07
TI  - Enhanced Milk-Clotting Enzyme Production of Mucor Miehei Mutated by UV and LiCl
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.62
DO  - https://doi.org/10.2991/bbe-16.2016.62
ID  - Zhang2016/07
ER  -