Age-related Senescence of Rat Bone Marrow-derived Mesenchymal Stem Cells
Authors
Xu He, Cao Ma, Chenchen Pi, Yue Yang, Lin Lin, Yingai Shi, Yulin Li
Corresponding Author
Xu He
Available Online July 2016.
- DOI
- https://doi.org/10.2991/bbe-16.2016.61How to use a DOI?
- Keywords
- MSCs, Cell Senescence, Aging.
- Abstract
- To study whether bone marrow-derived mesenchymal stem cells (MSCs) from aged rat present cell senescence. In the present study, cell morphology were observed, and cell proliferation and cell cycle were analyzed. The natural senescence of MSCs from aged rat was testified by investigation of SA- -gal activity, ROS levels, DNA damage, telomerase activity and senescence-associated factors. Our results demonstrated that MSCs from aged rat showed senescent morphology and cell growth slowed down. SA- -gal activity was elevated, the intracellular ROS was enhanced, cellular DNA was severely damaged, telomerase activity was declined, and the expression of senescence-associated factors pl6INK4Aand p21WAF1/ CIP were upregulated. Taken together, our findings reveal that MSCs display biological changes and natural senescence in the process of physiological individual aging.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Xu He AU - Cao Ma AU - Chenchen Pi AU - Yue Yang AU - Lin Lin AU - Yingai Shi AU - Yulin Li PY - 2016/07 DA - 2016/07 TI - Age-related Senescence of Rat Bone Marrow-derived Mesenchymal Stem Cells BT - International Conference on Biomedical and Biological Engineering PB - Atlantis Press SN - 2468-5747 UR - https://doi.org/10.2991/bbe-16.2016.61 DO - https://doi.org/10.2991/bbe-16.2016.61 ID - He2016/07 ER -