Age-related Senescence of Rat Bone Marrow-derived Mesenchymal Stem Cells

DOI

10.2991/bbe-16.2016.61How to use a DOI?

Keywords

MSCs, Cell Senescence, Aging.

Abstract

To study whether bone marrow-derived mesenchymal stem cells (MSCs) from aged rat present cell senescence. In the present study, cell morphology were observed, and cell proliferation and cell cycle were analyzed. The natural senescence of MSCs from aged rat was testified by investigation of SA- -gal activity, ROS levels, DNA damage, telomerase activity and senescence-associated factors. Our results demonstrated that MSCs from aged rat showed senescent morphology and cell growth slowed down. SA- -gal activity was elevated, the intracellular ROS was enhanced, cellular DNA was severely damaged, telomerase activity was declined, and the expression of senescence-associated factors pl6INK4Aand p21WAF1/ CIP were upregulated. Taken together, our findings reveal that MSCs display biological changes and natural senescence in the process of physiological individual aging.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Xu He
AU  - Cao Ma
AU  - Chenchen Pi
AU  - Yue Yang
AU  - Lin Lin
AU  - Yingai Shi
AU  - Yulin Li
PY  - 2016/07
DA  - 2016/07
TI  - Age-related Senescence of Rat Bone Marrow-derived Mesenchymal Stem Cells
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 399
EP  - 403
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.61
DO  - 10.2991/bbe-16.2016.61
ID  - He2016/07
ER  -