Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Selection of Potential Probiotic Strains Isolated from Human Intestinal Tract and Traditional Ferment Milk

Authors
Chaohui Xue, Chun Yue, Xueguo Liu, Le Yuan
Corresponding Author
Chaohui Xue
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.63How to use a DOI?
Keywords
Lactobacillus rhamnosus, Salmonella, Diarrhoea, Low pH, Bile salts.
Abstract
Diarrhea is the main reason of human deaths in developing countries, which also cause world-wide infant mortality. Diarrhoea occurs world-wide and causes 4% of all deaths and 5% of health loss to disability. It is most commonly caused by gastrointestinal infections which kill around 1.5 million people globally each year, mostly children in developing countries. In this study, five selected Lactobacillus isolated from human intestinal and ferment milk were preliminarily identified by 16S rDNA gene sequencing and assessed the ability to inhibit the infection of enteropathogens. The Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). Using an in vitro system simulating gastric transit, our findings indicated that the probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of pathogensto the HT-29 cell. Among the selected strains, the selected Lactobacillus rhamnosus F0533 showed a high probiotic potential and could be used in health-promoting food products.After analyzing the sequence of the 16SrDNA regions of these three strains, the potential probiotic F0533 were Lactobacillus rhamnosus.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.63How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Chaohui Xue
AU  - Chun Yue
AU  - Xueguo Liu
AU  - Le Yuan
PY  - 2016/07
DA  - 2016/07
TI  - Selection of Potential Probiotic Strains Isolated from Human Intestinal Tract and Traditional Ferment Milk
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.63
DO  - https://doi.org/10.2991/bbe-16.2016.63
ID  - Xue2016/07
ER  -